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rdf:type |
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lifeskim:mentions |
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pubmed:dateCreated |
1995-6-22
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pubmed:abstractText |
The 5' flanking region of the Clara cell secretory protein (CCSP) gene contains two cis-acting elements which bind hepatocyte nuclear factor (HNF)-3 alpha and HNF-3 beta in vitro. To determine the role of these proteins in mediating CCSP gene expression in the bronchiolar epithelium, chimeric CCSP-reporter gene constructs containing various regions of the CCSP 5' flanking region were co-transfected into H-441 cells with HNF-3 alpha or HNF-3 beta expression plasmids. These studies indicate that each of these transcription factors positively regulates CCSP gene expression and revealed that CCSP region I (-132 to -76) is sufficient to mediate this effect. Gel-mobility-shift assays with oligonucleotides corresponding to CCSP region I, nuclear extract from bronchiolar epithelial cells and HNF-3-specific antibodies indicate that HNF-3 alpha and HNF-3 beta are the only proteins in bronchiolar epithelial cells which directly interact with this region. Consistent with these observations, HNF-3 alpha and HNF-3 beta transcripts were found to be enriched in this cell population and in situ hybridization of adult lung revealed HNF-3 gene expression in non-ciliated bronchiolar epithelial cells expressing the CCSP gene. Finally, experiments with CCSP region I and a heterologous promoter indicate that this region acts in a promoter-specific context, suggesting that additional factors interacting via the minimal CCSP promoter region are essential in determining the effects of HNF-3 on cell-specific CCSP gene expression in the bronchiolar epithelium.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/7755566-1281842,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7755566-1409605,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7755566-1497309,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7755566-1505020,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7755566-1562179,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7755566-1566856,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7755566-1634515,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7755566-1730611,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7755566-1733279,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7755566-2115524,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7755566-2227418,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7755566-2349092,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7755566-2771659,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7755566-2837828,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7755566-518835,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7755566-6091052,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7755566-6418790,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7755566-7127302,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7755566-7916613,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7755566-7958446,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7755566-8020953,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7755566-8049073,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7755566-8065304,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7755566-8071346,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7755566-8187630,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7755566-8216221,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7755566-8248124,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7755566-8306889,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7755566-8321193,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7755566-8398159
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Foxa1 protein, rat,
http://linkedlifedata.com/resource/pubmed/chemical/Foxa2 protein, rat,
http://linkedlifedata.com/resource/pubmed/chemical/Hepatocyte Nuclear Factor 3-alpha,
http://linkedlifedata.com/resource/pubmed/chemical/Hepatocyte Nuclear Factor 3-beta,
http://linkedlifedata.com/resource/pubmed/chemical/Nuclear Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Scgb1a1 protein, rat,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors,
http://linkedlifedata.com/resource/pubmed/chemical/Uteroglobin
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0264-6021
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
308 ( Pt 1)
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pubmed:geneSymbol |
CCSP
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
197-202
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pubmed:dateRevised |
2011-11-17
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pubmed:meshHeading |
pubmed-meshheading:7755566-Animals,
pubmed-meshheading:7755566-Bronchi,
pubmed-meshheading:7755566-DNA-Binding Proteins,
pubmed-meshheading:7755566-Epithelium,
pubmed-meshheading:7755566-Gene Expression Regulation,
pubmed-meshheading:7755566-Hepatocyte Nuclear Factor 3-alpha,
pubmed-meshheading:7755566-Hepatocyte Nuclear Factor 3-beta,
pubmed-meshheading:7755566-In Situ Hybridization,
pubmed-meshheading:7755566-Nuclear Proteins,
pubmed-meshheading:7755566-Promoter Regions, Genetic,
pubmed-meshheading:7755566-Proteins,
pubmed-meshheading:7755566-RNA, Messenger,
pubmed-meshheading:7755566-Rats,
pubmed-meshheading:7755566-Transcription Factors,
pubmed-meshheading:7755566-Uteroglobin
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pubmed:year |
1995
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pubmed:articleTitle |
Role of hepatocyte nuclear factor-3 alpha and hepatocyte nuclear factor-3 beta in Clara cell secretory protein gene expression in the bronchiolar epithelium.
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pubmed:affiliation |
Edward Mallinckrodt Department of Pediatrics, Washington University School of Medicine, St. Louis, MO, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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