Linked Life Data

7755283

Source:http://linkedlifedata.com/resource/pubmed/id/7755283

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
1995-6-16
pubmed:abstractText
Increasing evidence suggests that angiotensin II may act as a growth factor for several muscle cell types. Angiotensin II stimulation activates many immediate early response genes like c-Fos, c-Jun, c-Myc and Egr-1 in both vascular smooth muscle cells and cardiomyocytes, independently of whether a hyperplastic or hypertrophic response is taking place. In this study we report that angiotensin II significantly stimulates AP1-driven transcription in mouse skeletal muscle cells C2C12 stably transfected with a TRE-tk-CAT plasmid in a dose-dependent manner (peak stimulation at 10(-5) M of angiotensin II). Moreover, angiotensin II increases the binding of the AP1 complex to its DNA target in both quiescent C2C12 myoblasts and in differentiated C2C12 myotubes. Most of the TRE-bound complexes in both unstimulated and angiotensin II-treated cells consist of c-jun/c-fos heterodimers. Using a set of different protein kinase inhibitors, including HA1004, H7, tyrphostin, genistein and staurosporine, we could demonstrate that the angiotensin II-induced AP1 binding increase is not mediated by the cAMP-dependent pathway and that protein kinase C and tyrosine kinases are involved. Treatment of C2C12 cells with H2O2 induces a dose-dependent increase in c-jun/c-fos heterodimer binding, specifically reverted by the cysteine derivative and glutathione precursor N-acetyl-L-cysteine (NAC). The observation that the induction by angiotensin II of both the AP1 DNA binding activity and DNA synthesis in quiescent C2C12 myoblasts is abolished by NAC strongly suggests a role for reactive oxygen intermediates (ROIs) in the intracellular transduction of angiotensin II signals for immediate early gene induction and for cell proliferation.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0077-8923
pubmed:author
pubmed:issnType
Print
pubmed:day
27
pubmed:volume
752
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
394-405
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed-meshheading:7755283-Angiotensin II, pubmed-meshheading:7755283-Animals, pubmed-meshheading:7755283-Antioxidants, pubmed-meshheading:7755283-Base Sequence, pubmed-meshheading:7755283-Cell Differentiation, pubmed-meshheading:7755283-Cell Line, pubmed-meshheading:7755283-Gene Expression, pubmed-meshheading:7755283-Genes, Immediate-Early, pubmed-meshheading:7755283-Heart, pubmed-meshheading:7755283-Mice, pubmed-meshheading:7755283-Molecular Sequence Data, pubmed-meshheading:7755283-Muscle, Skeletal, pubmed-meshheading:7755283-Muscle, Smooth, Vascular, pubmed-meshheading:7755283-Myocardium, pubmed-meshheading:7755283-Oligodeoxyribonucleotides, pubmed-meshheading:7755283-Protein Kinase Inhibitors, pubmed-meshheading:7755283-Protein Kinases, pubmed-meshheading:7755283-Proto-Oncogene Proteins c-fos, pubmed-meshheading:7755283-Proto-Oncogene Proteins c-jun, pubmed-meshheading:7755283-Reactive Oxygen Species, pubmed-meshheading:7755283-Recombinant Fusion Proteins, pubmed-meshheading:7755283-Signal Transduction, pubmed-meshheading:7755283-Transcription Factor AP-1, pubmed-meshheading:7755283-Transfection
pubmed:year
1995
pubmed:articleTitle
Reactive oxygen intermediates (ROIs) are involved in the intracellular transduction of angiotensin II signal in C2C12 cells.
pubmed:affiliation
Fondazione Andrea Cesalpino, University of Rome La Sapienza, Italy.
pubmed:publicationType
Journal Article, Review