Linked Life Data

7751810

Source:http://linkedlifedata.com/resource/pubmed/id/7751810

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1995-6-22
pubmed:abstractText
Total serum free fatty acids (FFAt) levels provide an important measure of the physiologic state. Most of the FFA in serum is bound to albumin; a small portion, however, is unbound. This study presents the first measurements of serum unbound free fatty acid (FFAu) concentrations. These measurements were made possible by the development of the fluorescence probe ADIFAB (acrylodated intestinal fatty acid binding protein). In the present study ADIFAB was shown to provide the correct value of the sum of the [FFAu] of each molecular species of long chain FFA: 1) in aqueous mixtures of FFA and ADIFAB; 2) in mixtures of FFA, human serum albumin, and ADIFAB; and 3) in serum and ADIFAB. Human serum [FFAu] were measured in 283 samples from healthy donors and yielded a mean value of 7.5 nM with a standard deviation of 2.5 nM. This measured value is as much as 1000-fold smaller than [FFAu] values previously estimated. The distribution of [FFAu] values was found to be invariant with donor gender and age. Average [FFAu] values do, as expected, exhibit a small (1.5 nM) but significant (P < 0.001) increase after overnight fasting. FFAu levels were found to be well correlated with total serum FFA or, equivalently, [FFAt]/[HSA]. Given their ease and accuracy, and because [FFAu] values increase exponentially with [FFAt]/[HSA], [FFAu] measurements could provide a sensitive method for assessing the physiologic state.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0022-2275
pubmed:author
pubmed:issnType
Print
pubmed:volume
36
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
229-40
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1995
pubmed:articleTitle
Unbound free fatty acid levels in human serum.
pubmed:affiliation
Medical Biology Institute, La Jolla, CA 92037, USA.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't