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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
11
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pubmed:dateCreated |
1995-6-22
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pubmed:abstractText |
Several cysteine and serine protease inhibitors previously shown to block TCR-induced death of 2B4 T hybridoma cells were tested for their ability to block various T lymphocyte apoptotic death systems. TCR-induced death of both peripheral CD4+ and CD8+ T cell blasts was inhibited similarly to the hybridoma, but cell death in these cells induced by anti-Fas, gamma-irradiation, etoposide, or extracellular ATP was not blocked. For T cell lines, cell death induced by CTL or by IL-2 withdrawal was also not inhibited. TCR-induced death of immature CD+8+ thymocytes triggered by culture on immobilized anti-CD3 was not blocked by these protease inhibitors, whereas similar death induced in the resting CD4+8- thymocyte subset under these conditions was inhibited similarly to the T cell blasts. TCR-induced proliferation of the latter subset was modest in the absence of exogeneous IL-2, but was enhanced two- to fourfold by the protease inhibitors. These results show that a protease-dependent death pathway can be triggered by the TCR in mature T cells; similar protease-dependent steps are not common to the TCR-triggered activation pathway or other apoptotic death pathways in these cells.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
AIM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0022-1767
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
154
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
5806-12
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pubmed:dateRevised |
2005-11-17
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pubmed:meshHeading |
pubmed-meshheading:7751630-Animals,
pubmed-meshheading:7751630-Antibodies, Monoclonal,
pubmed-meshheading:7751630-Apoptosis,
pubmed-meshheading:7751630-CD4-Positive T-Lymphocytes,
pubmed-meshheading:7751630-CD8-Positive T-Lymphocytes,
pubmed-meshheading:7751630-Calpain,
pubmed-meshheading:7751630-Cell Differentiation,
pubmed-meshheading:7751630-Cell Line,
pubmed-meshheading:7751630-Cytotoxicity, Immunologic,
pubmed-meshheading:7751630-Endopeptidases,
pubmed-meshheading:7751630-Leupeptins,
pubmed-meshheading:7751630-Lymph Nodes,
pubmed-meshheading:7751630-Male,
pubmed-meshheading:7751630-Mice,
pubmed-meshheading:7751630-Mice, Inbred C57BL,
pubmed-meshheading:7751630-Protease Inhibitors,
pubmed-meshheading:7751630-Receptors, Antigen, T-Cell,
pubmed-meshheading:7751630-T-Lymphocytes,
pubmed-meshheading:7751630-Thymus Gland
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pubmed:year |
1995
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pubmed:articleTitle |
A protease-dependent TCR-induced death pathway in mature lymphocytes.
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pubmed:affiliation |
Experimental Immunology Branch, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA.
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pubmed:publicationType |
Journal Article
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