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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1995-6-22
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pubmed:abstractText |
Peripheral blood mononuclear cells (PBMC) stimulated with pokeweed mitogen (PWM) produced increased IgG when cultured with interleukin-6 (IL-6). IgG subclass analysis showed that the presence of IL-6 during the last half of the culture period enhanced IgG1 and IgG4 production. Enhancement of IgG2 synthesis required the presence of IL-6 solely during the last half of the culture period, whereas enhancement paradoxically was blocked by its presence during the first half. The IgG3-enhancing effect of IL-6 was observed only when IL-6 was present throughout the culture period. The critical role of IL-6 was supported by the inhibition of IgG subclass synthesis by anti-IL-6 antibody. PBMC depleted of cells bearing surface IgG of a particular subclass did not synthesize that subclass. This non-responsiveness, which was not reversed even by an addition of IL-6, indicates that the main action of IL-6 is on the differentiation of committed B cells. In addition, IL-6 triggered T cells to produce significant helper activity. These results indicate that IL-6 enhances IgG subclass production differentially and that its critical role in IgG subclass synthesis is in part mediated by T cells, as well as by its direct action on B cells. These findings should be useful for analysing such immune disorders as IgG subclass deficiencies and autoimmune diseases.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/7751005-14186462,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7751005-14206440,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7751005-1711085,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7751005-1832176,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7751005-1997653,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7751005-2104575,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7751005-2107992,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7751005-2179132,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7751005-2295801,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7751005-233982,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7751005-2373516,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7751005-2410927,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7751005-2493049,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7751005-2788092,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7751005-2829354,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7751005-3258006,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7751005-3258060,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7751005-3311462,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7751005-3493321,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7751005-3499611,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7751005-3899923,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7751005-4179068,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7751005-6217248,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7751005-6311731,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7751005-6481161,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7751005-6610704,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7751005-7909497,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7751005-8020547,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7751005-8097323,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7751005-8294883,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7751005-8376788,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7751005-8396532
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0019-2805
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
84
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
278-84
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:7751005-Adult,
pubmed-meshheading:7751005-Antibodies, Anti-Idiotypic,
pubmed-meshheading:7751005-B-Lymphocytes,
pubmed-meshheading:7751005-Cells, Cultured,
pubmed-meshheading:7751005-Humans,
pubmed-meshheading:7751005-Immunoglobulin Class Switching,
pubmed-meshheading:7751005-Immunoglobulin G,
pubmed-meshheading:7751005-Interleukin-6,
pubmed-meshheading:7751005-Kinetics,
pubmed-meshheading:7751005-Pokeweed Mitogens,
pubmed-meshheading:7751005-Recombinant Proteins,
pubmed-meshheading:7751005-T-Lymphocytes
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pubmed:year |
1995
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pubmed:articleTitle |
Regulation of human IgG subclass production by cytokines: human IgG subclass production enhanced differentially by interleukin-6.
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pubmed:affiliation |
Department of Pediatrics, Saitama Medical School, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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