|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
1
|
|
pubmed:dateCreated |
1995-6-22
|
|
pubmed:abstractText |
A method has been developed for introducing heterologous DNA rapidly and efficiently by electropermeabilization into the yeast Schwanniomyces occidentalis. A transformation efficiency as high as 2 x 10(5) transformants/microgram of plasmid DNA was obtained with a square-wave electric pulse of 2.17 kV/cm during 18 ms. Small quantities of DNA (5 ng) can be used to transform 3 x 10(8) cells. The main parameters which have been optimized are: presence of adenine in the culture medium, pretreatment of the cells with dithiothreitol during the exponential growth phase of the cells, amount of cells treated, and pulse-field strength and duration. Competent cells can be stored to allow electrotransformation whenever needed.
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Dec
|
|
pubmed:issn |
0172-8083
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:volume |
27
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
26-30
|
|
pubmed:dateRevised |
2006-11-15
|
|
pubmed:meshHeading |
pubmed-meshheading:7750143-Adenine,
pubmed-meshheading:7750143-Cryopreservation,
pubmed-meshheading:7750143-Cryoprotective Agents,
pubmed-meshheading:7750143-Culture Media,
pubmed-meshheading:7750143-DNA, Fungal,
pubmed-meshheading:7750143-Dithiothreitol,
pubmed-meshheading:7750143-Electroporation,
pubmed-meshheading:7750143-Osmolar Concentration,
pubmed-meshheading:7750143-Plasmids,
pubmed-meshheading:7750143-Transformation, Genetic,
pubmed-meshheading:7750143-Yeasts
|
|
pubmed:year |
1994
|
|
pubmed:articleTitle |
High-efficiency electrotransformation of the yeast Schwanniomyces occidentalis.
|
|
pubmed:affiliation |
Institut National des Sciences Appliquées, CNRS, Toulouse, France.
|
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|
