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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
4
|
| pubmed:dateCreated |
1995-6-22
|
| pubmed:abstractText |
The recent identification of the sequences of the peptides derived from a number of human melanoma-associated antigens has presented opportunities for developing a specific-peptide-based vaccine in this form of cancer. Since antigen-presenting cells (APC) play a crucial role in the induction of the T-cell-mediated immune response, we examined whether or not ex vivo cultured APC, bearing the appropriate MHC restricting elements, when pulsed with a relevant melanoma-specific cytotoxic-T-lymphocyte (CTL)-determined peptide, can present the peptide to the CTL. Here we show that a population of cells, derived from the monocyte/macrophage lineage from peripheral blood and grown in granulocyte/macrophage-colony-stimulating factor, exhibit many essential characteristics of "professional" APC (dendritic-type morphology with a proportion of the population, the B7 molecule, and high levels of MHC class I and class II molecules, CD11b and CD54 molecules) and are capable of efficiently presenting the nonapeptide, EADPTGHSY, encoded by the melanoma antigen MAGE-1 gene, to the MAGE-1-specific CTL clone, 82/30. These results suggest that this type of autologous ex vivo cultured population of professional APC, when pulsed with the relevant-CTL-determined peptide, can serve as a novel type of candidate vaccine for active specific immunization against HLA-A1-positive patients with melanoma expressing the MAGE-1 antigen.
|
| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Neoplasm,
http://linkedlifedata.com/resource/pubmed/chemical/Granulocyte-Macrophage...,
http://linkedlifedata.com/resource/pubmed/chemical/Histocompatibility Antigens Class I,
http://linkedlifedata.com/resource/pubmed/chemical/MAGEA1 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Melanoma-Specific Antigens,
http://linkedlifedata.com/resource/pubmed/chemical/Neoplasm Proteins
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Apr
|
| pubmed:issn |
0340-7004
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
40
|
| pubmed:geneSymbol |
MAGE-1
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
268-71
|
| pubmed:dateRevised |
2011-11-17
|
| pubmed:meshHeading |
pubmed-meshheading:7750125-Amino Acid Sequence,
pubmed-meshheading:7750125-Antigen-Presenting Cells,
pubmed-meshheading:7750125-Antigens, Neoplasm,
pubmed-meshheading:7750125-Base Sequence,
pubmed-meshheading:7750125-Cells, Cultured,
pubmed-meshheading:7750125-Granulocyte-Macrophage Colony-Stimulating Factor,
pubmed-meshheading:7750125-Histocompatibility Antigens Class I,
pubmed-meshheading:7750125-Humans,
pubmed-meshheading:7750125-Lymphocyte Activation,
pubmed-meshheading:7750125-Macrophages,
pubmed-meshheading:7750125-Melanoma,
pubmed-meshheading:7750125-Melanoma-Specific Antigens,
pubmed-meshheading:7750125-Molecular Sequence Data,
pubmed-meshheading:7750125-Neoplasm Proteins,
pubmed-meshheading:7750125-Stimulation, Chemical,
pubmed-meshheading:7750125-T-Lymphocytes,
pubmed-meshheading:7750125-T-Lymphocytes, Cytotoxic,
pubmed-meshheading:7750125-Tumor Cells, Cultured
|
| pubmed:year |
1995
|
| pubmed:articleTitle |
Presentation of synthetic peptide antigen encoded by the MAGE-1 gene by granulocyte/macrophage-colony-stimulating-factor-cultured macrophages from HLA-A1 melanoma patients.
|
| pubmed:affiliation |
Department of Medicine, University of Connecticut Health Center, Farmington 06030-3210, USA.
|
| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
|