Linked Life Data

7749874

Source:http://linkedlifedata.com/resource/pubmed/id/7749874

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
1995-6-20
pubmed:abstractText
Macrophage scavenger receptors mediate the uptake of chemically modified LDL in an unregulated manner, leading to massive intracellular accumulation of lipid and thus a foamy cellular morphology. In atherosclerotic lesions, foam cells originate not only from macrophages but also from smooth muscle cells, yet smooth muscle cells do not normally express scavenger receptors, and when exposed to chemically modified LDL in vitro, lipid accumulation does not occur. The mechanism of conversion of smooth muscle cells into foam cells in the arterial wall is thus still under discussion. To investigate whether direct interaction between macrophages and smooth muscle cells may be involved and to explore the effects of components of the two cell types on the expression of scavenger receptors, we report here experiments using somatic cell hybrids formed by fusion of the two cell types. Immunofluorescent labeling and confocal microscopic techniques were applied to investigate and measure (1) lipid accumulation (using Nile Red staining), (2) the binding and uptake of acetylated LDL (using 1,1'-dioctadecyl-1-3,3,3',3'-tetramethyl-indocarbocyanine perchlorate-labeled acetylated LDL), and (3) receptor expression (assessed using a specific anti-receptor antibody) in smooth muscle cell-macrophage heterokaryons, macrophage-macrophage homokaryons, smooth muscle cell-smooth muscle cell homokaryons, and unfused macrophages and smooth muscle cells. The results demonstrate that scavenger receptor expression becomes repressed in macrophage-smooth muscle cell heterokaryons but not in macrophage-macrophage homokaryons. One possible explanation for the observed repression would be the existence of a negative regulatory cytoplasmic factor produced by smooth muscle cells.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
1079-5642
pubmed:author
pubmed:issnType
Print
pubmed:volume
15
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
601-11
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:7749874-Animals, pubmed-meshheading:7749874-Antibodies, Monoclonal, pubmed-meshheading:7749874-Cell Fusion, pubmed-meshheading:7749874-Female, pubmed-meshheading:7749874-Fluorescent Antibody Technique, pubmed-meshheading:7749874-Hybrid Cells, pubmed-meshheading:7749874-Lipid Metabolism, pubmed-meshheading:7749874-Lipoproteins, LDL, pubmed-meshheading:7749874-Macrophages, pubmed-meshheading:7749874-Membrane Proteins, pubmed-meshheading:7749874-Mice, pubmed-meshheading:7749874-Microscopy, Confocal, pubmed-meshheading:7749874-Muscle, Smooth, pubmed-meshheading:7749874-Receptors, Immunologic, pubmed-meshheading:7749874-Receptors, Lipoprotein, pubmed-meshheading:7749874-Receptors, Scavenger, pubmed-meshheading:7749874-Scavenger Receptors, Class B, pubmed-meshheading:7749874-Swine
pubmed:year
1995
pubmed:articleTitle
Repression of the macrophage scavenger receptor in macrophage-smooth muscle cell heterokaryons.
pubmed:affiliation
Institute for Arteriosclerosis Research, University of Münster, Germany.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't