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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions |
umls-concept:C0001613,
umls-concept:C0002246,
umls-concept:C0005456,
umls-concept:C0007776,
umls-concept:C0010453,
umls-concept:C0019564,
umls-concept:C0022655,
umls-concept:C0028040,
umls-concept:C0034693,
umls-concept:C0034721,
umls-concept:C0041904,
umls-concept:C0087111,
umls-concept:C0162493,
umls-concept:C0205191,
umls-concept:C0205265,
umls-concept:C1555582,
umls-concept:C1711351,
umls-concept:C2003941
|
| pubmed:issue |
1-2
|
| pubmed:dateCreated |
1995-6-22
|
| pubmed:abstractText |
High density neuronal cultures from rat E18 hippocampus and cortex have been characterised with respect to cholinergic binding sites. No specific binding of [3H]nicotine or [3H]cytisine to live cells in situ was detected although the limit for detection was estimated to be 30 fmol/mg protein. Muscarinic binding sites labelled with [3H]QNB were present at a density of 0.75 pmol/mg protein. [125I]alpha-Bungarotoxin (alpha Bgt) bound to hippocampal cultures with a Bmax of 128 fmol/mg protein and a Kd of 0.6 nM; cortical cultures expressed five times fewer [125I]alpha-Bgt binding sites. Fluorescence cytochemistry with rhodamine-alpha-Bgt indicated that 95% of hippocampal neurons were labelled, compared with only 36% of cortical neurons. Average densities of 4 x 10(4) and 2 x 10(4) binding sites/cell were calculated for hippocampal and cortical cultures, respectively. Double labelling experiments with mAb307 (which recognises the rat alpha 7 nicotinic receptor subunit) and rhodamine-alpha-Bgt gave coincident labelling patterns, supporting the correlation between the alpha 7 subunit and Bgt-sensitive neuronal nicotinic receptor. Treatment of hippocampal cultures with 10 microM nicotine for 14 days elicited a 40% increase in the numbers of [125I]alpha-Bgt binding sites, mimicking the up-regulation observed in in vivo studies. Primary cultures offer a useful in vitro system for investigating the expression and regulation of brain alpha-Bgt-sensitive receptors.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Feb
|
| pubmed:issn |
0006-8993
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
20
|
| pubmed:volume |
672
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
228-36
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:7749744-Animals,
pubmed-meshheading:7749744-Binding Sites,
pubmed-meshheading:7749744-Bungarotoxins,
pubmed-meshheading:7749744-Cells, Cultured,
pubmed-meshheading:7749744-Cerebral Cortex,
pubmed-meshheading:7749744-Hippocampus,
pubmed-meshheading:7749744-Immunohistochemistry,
pubmed-meshheading:7749744-Nicotine,
pubmed-meshheading:7749744-Quinuclidinyl Benzilate,
pubmed-meshheading:7749744-Rats,
pubmed-meshheading:7749744-Rats, Sprague-Dawley,
pubmed-meshheading:7749744-Receptors, Nicotinic,
pubmed-meshheading:7749744-Time Factors,
pubmed-meshheading:7749744-Tissue Distribution,
pubmed-meshheading:7749744-Up-Regulation
|
| pubmed:year |
1995
|
| pubmed:articleTitle |
alpha-Bungarotoxin binding sites in rat hippocampal and cortical cultures: initial characterisation, colocalisation with alpha 7 subunits and up-regulation by chronic nicotine treatment.
|
| pubmed:affiliation |
School of Biology and Biochemistry, University of Bath, UK.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|