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rdf:type |
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lifeskim:mentions |
umls-concept:C0014644,
umls-concept:C0017262,
umls-concept:C0021311,
umls-concept:C0040649,
umls-concept:C0086418,
umls-concept:C0185117,
umls-concept:C0521115,
umls-concept:C0814999,
umls-concept:C1336789,
umls-concept:C1515568,
umls-concept:C2911684
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pubmed:issue |
2
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pubmed:dateCreated |
1995-6-9
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pubmed:abstractText |
Epstein-Barr virus (EBV) is known to infect B cells and epithelial cells. We and others have shown that EBV can also infect a subset of thymocytes. Infection of thymocytes was accompanied by the appearance of linear EBV genome within 8 hr of infection. Circularization of the EBV genome was not detected. This is in contrast to the infection in B cells where the genome can circularize within 24 hr of infection. The appearance of the BamHI ZLF-1 gene product, ZEBRA, by RT-PCR, was observed within 8 hr of infection. The appearance of a novel fusion transcript (RAZ), which comprised regions of the BZLF-1 locus and the adjacent BRLF-1 locus, was detected by RT-PCR. ZEBRA protein was also identified in infected thymocytes by immunoprecipitation. In addition, we demonstrated that the EBNA-1 gene in infected thymocytes was transcribed from the Fp promoter, rather than from the Cp/Wp promoter which is used in latently infected B cells. Transcripts encoding gp350/220, the major coat protein of EBV, were identified, but we did not find any evidence of transcription from the LMP-2A or EBER-1 loci in infected thymocytes. These observations suggest that de novo EBV infection of thymocytes differs from infection of B cells. The main difference is that with thymocytes, no evidence could be found that the virus ever circularizes. Rather, EBV remains in a linear configuration from which replicative genes are transcribed.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Viral,
http://linkedlifedata.com/resource/pubmed/chemical/BRLF1 protein, Human herpesvirus 4,
http://linkedlifedata.com/resource/pubmed/chemical/BZLF1 protein, Herpesvirus 4, Human,
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Viral,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Epstein-Barr Virus Nuclear Antigens,
http://linkedlifedata.com/resource/pubmed/chemical/Immediate-Early Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Viral,
http://linkedlifedata.com/resource/pubmed/chemical/Repressor Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Trans-Activators,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors,
http://linkedlifedata.com/resource/pubmed/chemical/Viral Proteins
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0042-6822
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
20
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pubmed:volume |
208
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
685-95
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:7747440-Antigens, Viral,
pubmed-meshheading:7747440-Cells, Cultured,
pubmed-meshheading:7747440-DNA, Viral,
pubmed-meshheading:7747440-DNA-Binding Proteins,
pubmed-meshheading:7747440-Epstein-Barr Virus Nuclear Antigens,
pubmed-meshheading:7747440-Gene Expression Regulation, Viral,
pubmed-meshheading:7747440-Genes, Viral,
pubmed-meshheading:7747440-Herpesvirus 4, Human,
pubmed-meshheading:7747440-Humans,
pubmed-meshheading:7747440-Immediate-Early Proteins,
pubmed-meshheading:7747440-Nucleic Acid Conformation,
pubmed-meshheading:7747440-Promoter Regions, Genetic,
pubmed-meshheading:7747440-RNA, Messenger,
pubmed-meshheading:7747440-RNA, Viral,
pubmed-meshheading:7747440-Repressor Proteins,
pubmed-meshheading:7747440-T-Lymphocytes,
pubmed-meshheading:7747440-Trans-Activators,
pubmed-meshheading:7747440-Transcription, Genetic,
pubmed-meshheading:7747440-Transcription Factors,
pubmed-meshheading:7747440-Viral Proteins,
pubmed-meshheading:7747440-Virus Latency,
pubmed-meshheading:7747440-Virus Replication
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pubmed:year |
1995
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pubmed:articleTitle |
Epstein-Barr virus replicative gene transcription during de novo infection of human thymocytes: simultaneous early expression of BZLF-1 and its repressor RAZ.
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pubmed:affiliation |
Department of Pediatrics, National Jewish Center for Immunology, Denver, Colorado 80206, USA.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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