Linked Life Data

7745290

Source:http://linkedlifedata.com/resource/pubmed/id/7745290

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1995-6-9
pubmed:abstractText
Polymerase chain reaction (PCR) has been extensively used for diagnosis recently because of its very high sensitivity and specificity. We studied the applicability of PCR to the early diagnosis of toxoplasmosis in a murine model orally infected with Toxoplasma gondii (S-273). PCR was performed using EH24 and HE27 primers synthesized by the phosphoramidite method. Mice blood and brains collected on various post infection days (PID) were analysed by PCR (35 cycles). A portion of the brain tissue from each mouse was examined microscopically for the presence of parasite cysts. Blood and brain PCR were positive on the 9th and 12th day post-infection (DPI). Toxoplasma cysts in brain tissue appeared only on the 18th PID. The results showed that Toxoplasma parasites can be detected earlier in the blood than in the brain during primary infection, indicating that blood PCR is the more useful procedure.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0387-5911
pubmed:author
pubmed:issnType
Print
pubmed:volume
69
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
158-63
pubmed:dateRevised
2008-12-16
pubmed:meshHeading
pubmed:year
1995
pubmed:articleTitle
The use of PCR in detecting toxoplasma parasites in the blood and brains of mice experimentally infected with Toxoplasma gondii.
pubmed:affiliation
Department of Clinical Pathology, Hyogo College of Medicine.
pubmed:publicationType
Journal Article