Linked Life Data

7743941

Source:http://linkedlifedata.com/resource/pubmed/id/7743941

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
1995-6-12
pubmed:abstractText
In order to study the regional specification of neural tissue we isolated Xotx2, a Xenopus homolog of the Drosophila orthodenticle gene. Xotx2 is initially expressed in Spemann's organizer and its expression is absent in the ectoderm of early gastrulae. As gastrulation proceeds, Xotx2 expression is induced in the overlying ectoderm and this domain of expression moves anteriorly in register with underlying anterior mesoderm throughout the remainder of gastrulation. The expression pattern of Xotx2 suggests that a wave of Xotx2 expression (marking anterior neurectoderm) travels through the ectoderm of the gastrula with the movement of underlying anterior (prechordal plate) mesoderm. This expression of Xotx2 is reminiscent of the Eyal-Giladi model for neural induction. According to this model, anterior neural-inducing signals emanating from underlying anterior mesoderm transiently induce anterior neural tissues after vertical contact with the overlying ectoderm. Further patterning is achieved when the ectoderm receives caudalizing signals as it comes in contact with more posterior mesoderm during subsequent gastrulation movements. Functional characterization of the Xotx2 protein has revealed its involvement in differentiation of the anterior-most tissue, the cement gland. Ectopic expression of Xotx2 in embryos induces extra cement glands in the skin as well as inducing a cement gland marker (XAG1) in isolated animal cap ectoderm. Microinjection of RNA encoding the organizer-specific homeo-domain protein goosecoid into the ventral marginal zone results in induction of the Xotx2 gene. This result, taken in combination with the indistinguishable expression patterns of Xotx2 and goosecoid in the anterior mesoderm suggests that Xotx2 is a target of goosecoid regulation.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0950-1991
pubmed:author
pubmed:issnType
Print
pubmed:volume
121
pubmed:geneSymbol
Xotx2
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
993-1004
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed-meshheading:7743941-Animals, pubmed-meshheading:7743941-Base Sequence, pubmed-meshheading:7743941-Blotting, Northern, pubmed-meshheading:7743941-Ectoderm, pubmed-meshheading:7743941-Embryonic Induction, pubmed-meshheading:7743941-Gastrula, pubmed-meshheading:7743941-Gene Expression, pubmed-meshheading:7743941-Gene Expression Regulation, Developmental, pubmed-meshheading:7743941-Genes, Homeobox, pubmed-meshheading:7743941-Homeodomain Proteins, pubmed-meshheading:7743941-In Situ Hybridization, pubmed-meshheading:7743941-Molecular Sequence Data, pubmed-meshheading:7743941-Morphogenesis, pubmed-meshheading:7743941-Nerve Tissue Proteins, pubmed-meshheading:7743941-Nervous System, pubmed-meshheading:7743941-Oligonucleotide Probes, pubmed-meshheading:7743941-Otx Transcription Factors, pubmed-meshheading:7743941-Trans-Activators, pubmed-meshheading:7743941-Xenopus, pubmed-meshheading:7743941-Xenopus Proteins
pubmed:year
1995
pubmed:articleTitle
Anterior neurectoderm is progressively induced during gastrulation: the role of the Xenopus homeobox gene orthodenticle.
pubmed:affiliation
Department of Developmental and Cell Biology, University of California, Irvine 92717-2300, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't