Linked Life Data

7743493

Source:http://linkedlifedata.com/resource/pubmed/id/7743493

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
10
pubmed:dateCreated
1995-6-12
pubmed:abstractText
Native human mammary MCF-7 adenocarcinoma cells and a subline displaying resistance to 4-hydroperoxycyclophosphamide, the chemically activated form of cyclophosphamide, were grown as multicellular spheroids or on a collagen sponge matrix and perfused for study by 31P and 13C nuclear magnetic resonance spectroscopy. The natural abundance 13C spectrum of the perfused cells exhibits well-resolved resonances due to the intracellular glutathione (GSH). The resistant cell line shows a higher intensity of the GSH 13C resonances, consistent with the increased GSH concentration determined from biochemical assays of extracts. Treatment of the resistant cell line with buthionine sulfoximine selectively diminishes the intensity of the GSH resonances in the 13C nuclear magnetic resonance spectrum.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0008-5472
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
55
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2012-6
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1995
pubmed:articleTitle
Noninvasive detection of elevated glutathione levels in MCF-7 cells resistant to 4-hydroperoxycyclophosphamide.
pubmed:affiliation
Department of Radiology, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.