Linked Life Data

7743324

Source:http://linkedlifedata.com/resource/pubmed/id/7743324

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1995-6-15
pubmed:abstractText
The contribution of microcirculatory failure to ischemia/reperfusion injury in isolated perfused rat livers was investigated using intravital epifluorescence videomicroscopy. The degree of microvascular shut-down during reperfusion was modulated by the reperfusion conditions: flow-controlled (10 ml/min), in which microcirculatory failure is minimized by maintenance of constant flow through the liver, and pressure-controlled, in which microvascular shut-down is allowed to occur. Livers underwent 60 min of ischemia, 90 min of ischemia, or no ischemia (control). Perfused sinusoids and dead hepatocytes were quantified in 10 standardized microscopic fields (9000 microns2) per liver during off-line video playback. With flow-controlled reperfusion, microvascular (sinusoid) shut-down was largely avoided; a maximum of 21% of the sinusoids failed to conduct flow. Pressure-controlled reperfusion, however, resulted in early and severe shut-down. A significant decrease of approximately 20-30% was found after 60 min of ischemia and 30 min of reperfusion, while, after 90-min ischemia and 90-min reperfusion, 90% of the sinusoids failed to conduct flow. The appearance of dead hepatocytes correlated well with the number of perfused sinusoids (r = -0.78 for flow controlled, r = -0.97 for pressure-controlled). Only an occasional dead hepatocyte was observed with control perfusion, while up to 50% stained with propidium iodide following 90-min ischemia and 90-min reperfusion under pressure-controlled conditions. These results indicate that loss of sinusoidal flow can be ameliorated by flow-controlled reperfusion; moreover, hepatocyte necrosis during reperfusion is highly dependent upon the integrity of the microcirculation.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
1073-2322
pubmed:author
pubmed:issnType
Print
pubmed:volume
1
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
3-9
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1994
pubmed:articleTitle
Microcirculatory failure determines lethal hepatocyte injury in ischemic/reperfused rat livers.
pubmed:affiliation
Department of Surgery, Johns Hopkins University School of Medicine, Baltimore, Maryland 21287, USA.
pubmed:publicationType
Journal Article, In Vitro, Research Support, U.S. Gov't, P.H.S.