Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1995-6-5
pubmed:abstractText
Culturing hepatocytes on different extracellular matrix (ECM) substrata including tissue culture plastic, type I collagen, Engelbreth-Holm-Swarm (EHS) gel and poly-N-p-vinylbenzyl-D-lactonamide (PVLA) regulated levels of mRNAs for cytoskeleton and liver-specific genes. In hepatocytes on EHS gel, the ratio of albumin/beta-actin in mRNA levels was high and serially increased during the culture period, while the ratio was low and declined in cells on plastic substratum, collagen or PVLA. The changes in cellular levels of albumin mRNA which were regulated by ECM corresponded with those in two liver-specific transcription factors, hepatocyte nuclear factors-1 and -4, which control the transcription of liver-specific genes. These results suggest that cell-matrix interaction may determine and maintain the differentiated phenotype of hepatocytes by regulating liver-specific transcription factors.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
http://linkedlifedata.com/resource/pubmed/chemical/Actins, http://linkedlifedata.com/resource/pubmed/chemical/Albumins, http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Hepatocyte Nuclear Factor 1, http://linkedlifedata.com/resource/pubmed/chemical/Hepatocyte Nuclear Factor 1-alpha, http://linkedlifedata.com/resource/pubmed/chemical/Hepatocyte Nuclear Factor 1-beta, http://linkedlifedata.com/resource/pubmed/chemical/Hepatocyte Nuclear Factor 4, http://linkedlifedata.com/resource/pubmed/chemical/Hnf1a protein, rat, http://linkedlifedata.com/resource/pubmed/chemical/Nuclear Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Phosphoproteins, http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger, http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0006-291X
pubmed:author
pubmed:issnType
Print
pubmed:day
5
pubmed:volume
210
pubmed:geneSymbol
TTR
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
38-43
pubmed:dateRevised
2009-11-24
pubmed:meshHeading
pubmed-meshheading:7741747-Actins, pubmed-meshheading:7741747-Albumins, pubmed-meshheading:7741747-Animals, pubmed-meshheading:7741747-Cells, Cultured, pubmed-meshheading:7741747-DNA-Binding Proteins, pubmed-meshheading:7741747-Extracellular Matrix, pubmed-meshheading:7741747-Gene Expression, pubmed-meshheading:7741747-Gene Expression Regulation, pubmed-meshheading:7741747-Hepatocyte Nuclear Factor 1, pubmed-meshheading:7741747-Hepatocyte Nuclear Factor 1-alpha, pubmed-meshheading:7741747-Hepatocyte Nuclear Factor 1-beta, pubmed-meshheading:7741747-Hepatocyte Nuclear Factor 4, pubmed-meshheading:7741747-Liver, pubmed-meshheading:7741747-Male, pubmed-meshheading:7741747-Nuclear Proteins, pubmed-meshheading:7741747-Phosphoproteins, pubmed-meshheading:7741747-RNA, Messenger, pubmed-meshheading:7741747-Rats, pubmed-meshheading:7741747-Rats, Wistar, pubmed-meshheading:7741747-Signal Transduction, pubmed-meshheading:7741747-Time Factors, pubmed-meshheading:7741747-Transcription Factors
pubmed:year
1995
pubmed:articleTitle
Regulation of hepatic genes and liver transcription factors in rat hepatocytes by extracellular matrix.
pubmed:affiliation
First Department of Internal Medicine, Gifu University School of Medicine, Japan.
pubmed:publicationType
Journal Article, In Vitro, Research Support, Non-U.S. Gov't