Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
|
| pubmed:dateCreated |
1995-6-5
|
| pubmed:abstractText |
In order to investigate the influence of loss of heterozygosity (LOH) events on mutation rate, we studied two closely related human lymphoblastoid cell lines, AHH-1 (h2E1.v2) and MCL-5, which are heterozygous at the tk locus (chromosome 17q23-25). Although they have similar mutant fractions at the hprt locus, the mutant fraction and rate at tk is four to five times higher in AHH-1. Analysis of 58 spontaneous TK- mutants from AHH-1 and MCL-5 showed that the occurrence of LOH events was more frequent (23/24) in AHH-1 than MCL-5 (16/34). A set of five microsatellite polymorphism loci was used to map the extent of LOH along chromosome 17q. In AHH-1 cells, 15/23 of the LOH events encompassed at least 35% of the sex-averaged genetic length of chromosome 17q (98 cM). Additionally, the next most extensive category of LOH accounted for 5/23 TK- mutants, and encompassed at least 17 cM. In contrast, LOH events observed in MCL-5 are very restricted in extent; only one LOH tract extended as far as 4 cM from tk. The higher mutation rate at tk in AHH-1 can, therefore, be entirely attributed to the recovery of chromosomal scale LOH in viable, normal growth TK- mutants. Furthermore, these data demonstrate that the regional potential for LOH is likely to be an important determinant of mutation rate for loci within that chromosomal segment.
|
| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Aminacrine,
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Satellite,
http://linkedlifedata.com/resource/pubmed/chemical/Hypoxanthine...,
http://linkedlifedata.com/resource/pubmed/chemical/Mutagens,
http://linkedlifedata.com/resource/pubmed/chemical/Nitrogen Mustard Compounds,
http://linkedlifedata.com/resource/pubmed/chemical/Thymidine Kinase,
http://linkedlifedata.com/resource/pubmed/chemical/acridine half-mustard
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Jan
|
| pubmed:issn |
0267-8357
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
10
|
| pubmed:geneSymbol |
hprt
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
53-8
|
| pubmed:dateRevised |
2007-11-14
|
| pubmed:meshHeading |
pubmed-meshheading:7739402-Aminacrine,
pubmed-meshheading:7739402-Amino Acid Sequence,
pubmed-meshheading:7739402-Base Sequence,
pubmed-meshheading:7739402-Cell Line,
pubmed-meshheading:7739402-Chromosomes, Human, Pair 17,
pubmed-meshheading:7739402-DNA, Satellite,
pubmed-meshheading:7739402-Frameshift Mutation,
pubmed-meshheading:7739402-Genotype,
pubmed-meshheading:7739402-Humans,
pubmed-meshheading:7739402-Hypoxanthine Phosphoribosyltransferase,
pubmed-meshheading:7739402-Lymphocytes,
pubmed-meshheading:7739402-Molecular Sequence Data,
pubmed-meshheading:7739402-Mutagenesis,
pubmed-meshheading:7739402-Mutagens,
pubmed-meshheading:7739402-Mutation,
pubmed-meshheading:7739402-Nitrogen Mustard Compounds,
pubmed-meshheading:7739402-Polymorphism, Genetic,
pubmed-meshheading:7739402-Thymidine Kinase,
pubmed-meshheading:7739402-Transfection
|
| pubmed:year |
1995
|
| pubmed:articleTitle |
Extensive loss of heterozygosity accounts for differential mutation rate on chromosome 17q in human lymphoblasts.
|
| pubmed:affiliation |
Environmental Toxicology Graduate Program, University of California, Riverside 92521, USA.
|
| pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
|