Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1995-5-30
pubmed:abstractText
Chicken liver 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase was expressed in E. coli by using a pET3a T7 RNA polymerase-based expression system and was purified to homogeneity. The kinase and bisphosphatase of the expressed bifunctional enzyme had kinetic properties identical to those of the native chicken liver enzyme. However, the kinase activity of the chicken liver enzyme was 7-fold higher, while the bisphosphatase activity was 50 percent lower than those of the rat liver enzyme. Cys-256 of the rat liver bisphosphatase domain is not conserved in the chicken liver enzyme. A site-directed mutation was engineered at Cys-256 of the rat liver enzyme and the results indicate that the variation of this residue is not responsible for the difference in fructose-2,6-bisphosphatase activity between the rat and chicken liver enzymes. It is postulated that the difference in the kinase/bisphosphatase activity ratios of these two enzymes results from differences in their NH2-terminal regions.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0006-291X
pubmed:author
pubmed:issnType
Print
pubmed:day
26
pubmed:volume
209
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
883-93
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed-meshheading:7733980-Animals, pubmed-meshheading:7733980-Base Sequence, pubmed-meshheading:7733980-Chickens, pubmed-meshheading:7733980-Chromatography, Ion Exchange, pubmed-meshheading:7733980-Cloning, Molecular, pubmed-meshheading:7733980-Codon, pubmed-meshheading:7733980-DNA Primers, pubmed-meshheading:7733980-Electrophoresis, Polyacrylamide Gel, pubmed-meshheading:7733980-Escherichia coli, pubmed-meshheading:7733980-Gene Expression, pubmed-meshheading:7733980-Kinetics, pubmed-meshheading:7733980-Liver, pubmed-meshheading:7733980-Magnesium, pubmed-meshheading:7733980-Molecular Sequence Data, pubmed-meshheading:7733980-Molecular Weight, pubmed-meshheading:7733980-Multienzyme Complexes, pubmed-meshheading:7733980-Mutagenesis, Site-Directed, pubmed-meshheading:7733980-Phosphofructokinase-2, pubmed-meshheading:7733980-Phosphoric Monoester Hydrolases, pubmed-meshheading:7733980-Phosphotransferases, pubmed-meshheading:7733980-Plasmids, pubmed-meshheading:7733980-Polymerase Chain Reaction, pubmed-meshheading:7733980-Rats, pubmed-meshheading:7733980-Recombinant Proteins, pubmed-meshheading:7733980-Restriction Mapping
pubmed:year
1995
pubmed:articleTitle
Expression of chicken liver 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase in Escherichia coli.
pubmed:affiliation
Shanghai Institute of Biochemistry, Academia Sinica, China.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't