Linked Life Data

7730664

Source:http://linkedlifedata.com/resource/pubmed/id/7730664

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1995-5-30
pubmed:abstractText
A novel sub-clone of the B9 hybridoma cell line (B9-1-3) has been selected by cloning following continuous culture in rhIL-13. This cell line shows an increased sensitivity to both hIL-13 and mIL-4 compared to the parental B9 cell line. The proliferative response to IL-13 can be blocked with an anti-IL-4 receptor monoclonal antibody but not with the soluble IL-4 receptor, suggesting that IL-13- and IL-4-binding receptor subunits are distinct but form part of a common receptor complex. Although the B9-1-3 cell line is still sensitive to picogrammes of IL-6, it can be used to measure IL-13 in the presence of IL-6 by inclusion of excess neutralizing IL-6 antibody. This cell line should thus prove useful both in measuring the IL-13 bioactivity and for the dissection of the molecular nature of the IL-13:IL-4 receptor complex.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0022-1759
pubmed:author
pubmed:issnType
Print
pubmed:day
12
pubmed:volume
181
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
29-36
pubmed:dateRevised
2003-11-14
pubmed:meshHeading
pubmed:year
1995
pubmed:articleTitle
Isolation of an IL-13-dependent subclone of the B9 cell line useful for the estimation of human IL-13 bioactivity.
pubmed:affiliation
Sanofi Recherche, Centre de Labège, France.
pubmed:publicationType
Journal Article