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rdf:type |
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lifeskim:mentions |
umls-concept:C0006556,
umls-concept:C0011860,
umls-concept:C0017262,
umls-concept:C0017337,
umls-concept:C0023745,
umls-concept:C0030281,
umls-concept:C0033684,
umls-concept:C0086418,
umls-concept:C0204727,
umls-concept:C0205409,
umls-concept:C0475264,
umls-concept:C0679058,
umls-concept:C1171362,
umls-concept:C1515670,
umls-concept:C1521079,
umls-concept:C1521913,
umls-concept:C1522642,
umls-concept:C1547699,
umls-concept:C2603343,
umls-concept:C2700640
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pubmed:issue |
5
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pubmed:dateCreated |
1995-5-31
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pubmed:databankReference |
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pubmed:abstractText |
The metabolism of glucose in insulin-secreting cells leads to closure of ATP-sensitive K+ channels (KATP), an event that initiates the insulin secretory process. Defects in insulin secretion are a common feature of non-insulin-dependent diabetes mellitus (NIDDM), and the beta-cell KATP that couples metabolism and membrane potential is a candidate for contributing to the development of this clinically and genetically heterogeneous disorder. We screened a hamster insulinoma cDNA library by low-stringency hybridization with a probe coding for the G-protein-coupled inwardly rectifying K+ channel GIRK1/KGA and isolated clones encoding a protein, KATP-2, whose sequence is 90% similar to that of the recently described KATP-1, an ATP-sensitive K+ channel expressed in heart and other tissues. RNA blotting showed that KATP mRNA was present in insulin-secreting cells and brain but not in heart. To assess the contribution of KATP-2 to the development of NIDDM, the human KATP-2 gene (symbol KCNJ7) was isolated and mapped to chromosome band 21q22.1 by fluorescence in situ hybridization. A simple tandem repeat DNA polymorphism, D21S1255, was identified in the region of the KATP-2 gene, and linkage studies between this marker and NIDDM were carried out in a group of Mexican-American sib pairs with NIDDM. There was no evidence for linkage between D21S1255 and NIDDM, indicating that KATP-2 is not a major susceptibility gene in this population.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
AIM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0012-1797
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
44
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pubmed:geneSymbol |
K<down>ATP</down>-2
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pubmed:owner |
NLM
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pubmed:authorsComplete |
N
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pubmed:pagination |
592-6
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pubmed:dateRevised |
2011-11-17
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pubmed:meshHeading |
pubmed-meshheading:7729621-Adenosine Triphosphate,
pubmed-meshheading:7729621-Amino Acid Sequence,
pubmed-meshheading:7729621-Animals,
pubmed-meshheading:7729621-Base Sequence,
pubmed-meshheading:7729621-Chromosome Mapping,
pubmed-meshheading:7729621-Chromosomes, Artificial, Yeast,
pubmed-meshheading:7729621-Chromosomes, Human, Pair 21,
pubmed-meshheading:7729621-Cricetinae,
pubmed-meshheading:7729621-DNA, Complementary,
pubmed-meshheading:7729621-DNA Primers,
pubmed-meshheading:7729621-Diabetes Mellitus, Type 2,
pubmed-meshheading:7729621-Genetic Linkage,
pubmed-meshheading:7729621-Humans,
pubmed-meshheading:7729621-In Situ Hybridization, Fluorescence,
pubmed-meshheading:7729621-Insulin,
pubmed-meshheading:7729621-Islets of Langerhans,
pubmed-meshheading:7729621-Molecular Sequence Data,
pubmed-meshheading:7729621-Potassium Channels,
pubmed-meshheading:7729621-RNA, Messenger,
pubmed-meshheading:7729621-Tissue Distribution,
pubmed-meshheading:7729621-Tumor Cells, Cultured
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pubmed:year |
1995
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pubmed:articleTitle |
Isolation of a cDNA clone encoding a KATP channel-like protein expressed in insulin-secreting cells, localization of the human gene to chromosome band 21q22.1, and linkage studies with NIDDM.
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pubmed:affiliation |
Howard Hughes Medical Institute, University of California, San Francisco, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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