Linked Life Data

7729481

Source:http://linkedlifedata.com/resource/pubmed/id/7729481

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1995-5-30
pubmed:abstractText
Pf60.1, a marker recently isolated from the human malaria parasite Plasmodium falciparum, defines a large multigene family encoding antigens of 60 kDa, expressed by the blood stages (Carcy et al., Molecular and Biochemical Parasitology, 1994, 68, 221-233). Southern blotting showed that DNA from all strains and field isolates analyzed contained a large number of Pf60.1 copies. Considerable RFLP was observed. This diversity could be likewise visualized by analyzing PCR fragments amplified using primers derived from the Pf60.1 insert. Specific, multiple-band patterns were generated from laboratory strains, cloned lines, or wild isolates. This was further outlined after RsaI digestion of the PCR products. The sensitivity of this amplification was such that products could be visualized using a DNA amount representing less than one genome equivalent. Moreover, amplification was observed in some strains using a single primer, suggesting that some members of the Pf60.1 family are adjacent in an inverted orientation. This analysis confirmed the genetic similarity of a subset of laboratory strains. The results described here show that the extended diversity of this P. falciparum gene family provides a useful and sensitive PCR approach for strain typing.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0014-4894
pubmed:author
pubmed:issnType
Print
pubmed:volume
80
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
463-72
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1995
pubmed:articleTitle
Plasmodium falciparum: typing of malaria parasites based on polymorphism of a novel multigene family.
pubmed:affiliation
Muséum National d'Histoire Naturelle, UA CNRS No. 114, Paris, France.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't