Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
4
|
| pubmed:dateCreated |
1995-5-30
|
| pubmed:abstractText |
The present study was aimed at verifying the occurrence, if any, of in vivo oxidative DNA damage in FA homozygotes, their parents and siblings. 8-Hydroxy-2'-deoxyguanosine (8-OHdG) was measured, by HPLC/EC, in DNA from circulating blood leucocytes from FA homozygotes and their relatives and compared with a group of paediatric and adult healthy subjects. The population studied consisted of: (i) 15 FA homozygotes; (ii) 24 FA heterozygotes; (iii) 11 siblings. The 8-OHdG level in FA homozygotes was significantly higher with respect to age-matched controls, with a mean level of 33.3 +/- 6.8 (mean +/- SE) and 3.9 +/- 0.26 8-OHdG/10(5) dG respectively. The FA parents (heterozygotes) also displayed higher 8-OHdG levels relative to controls. The release of hydroxyl (.OH) and .OH-like radicals from leucocytes was determined by luminol-dependent chemiluminescence (LDCL) in a subgroup of FA homo- and heterozygotes, showing a very large in vivo formation of non-superoxide radicals. Chromosomal instability was also measured in the FA population. When relating either 8-OHdG or LDCL levels to spontaneous or diepoxybutane-induced chromosomal instability (S-CI and DEB-CI respectively), a significant correlation was observed between the 8-OHdG, LDCL and S-CI data. Within families a positive association was found between 8-OHdG levels in homozygotes and their related heterozygotes, suggesting segregation of the genetic defect(s) underlying the abnormal oxidative metabolism. The present study provides evidence for an in vivo pro-oxidant state in FA, in terms of excess formation of .OH and .OH-like radicals, and of DNA hydroxyl adducts. This finding appears to be shared by homozygotes and, to a lesser extent, by heterozygotes.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/8-hydroxy-2'-deoxyguanosine,
http://linkedlifedata.com/resource/pubmed/chemical/DNA,
http://linkedlifedata.com/resource/pubmed/chemical/Deoxyguanosine,
http://linkedlifedata.com/resource/pubmed/chemical/Epoxy Compounds,
http://linkedlifedata.com/resource/pubmed/chemical/Luminol,
http://linkedlifedata.com/resource/pubmed/chemical/Reactive Oxygen Species,
http://linkedlifedata.com/resource/pubmed/chemical/erythritol anhydride
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Apr
|
| pubmed:issn |
0143-3334
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
16
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
735-41
|
| pubmed:dateRevised |
2011-11-17
|
| pubmed:meshHeading |
pubmed-meshheading:7728950-Adolescent,
pubmed-meshheading:7728950-Adult,
pubmed-meshheading:7728950-Aged,
pubmed-meshheading:7728950-Aged, 80 and over,
pubmed-meshheading:7728950-Child,
pubmed-meshheading:7728950-Child, Preschool,
pubmed-meshheading:7728950-Chromatography, High Pressure Liquid,
pubmed-meshheading:7728950-Chromosomes, Human,
pubmed-meshheading:7728950-DNA,
pubmed-meshheading:7728950-DNA Damage,
pubmed-meshheading:7728950-Deoxyguanosine,
pubmed-meshheading:7728950-Epoxy Compounds,
pubmed-meshheading:7728950-Fanconi Anemia,
pubmed-meshheading:7728950-Female,
pubmed-meshheading:7728950-Heterozygote,
pubmed-meshheading:7728950-Homozygote,
pubmed-meshheading:7728950-Humans,
pubmed-meshheading:7728950-Leukocytes,
pubmed-meshheading:7728950-Luminescent Measurements,
pubmed-meshheading:7728950-Luminol,
pubmed-meshheading:7728950-Male,
pubmed-meshheading:7728950-Middle Aged,
pubmed-meshheading:7728950-Oxidation-Reduction,
pubmed-meshheading:7728950-Reactive Oxygen Species,
pubmed-meshheading:7728950-Respiratory Burst
|
| pubmed:year |
1995
|
| pubmed:articleTitle |
In vivo accumulation of 8-hydroxy-2'-deoxyguanosine in DNA correlates with release of reactive oxygen species in Fanconi's anaemia families.
|
| pubmed:affiliation |
IST, Istituto Nazionale per la Ricerca sul Cancro, Genoa, Italy.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|