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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
2-3
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pubmed:dateCreated |
1995-5-31
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pubmed:databankReference | |
pubmed:abstractText |
cDNA generated from stimulated abomasal lymph node cells was used to amplify and clone the 35 kDa and 40 kDa subunits of bovine interleukin 12 (IL-12) using primers derived from semi-conserved regions between human and mouse IL-12 sequences. The deduced amino acid sequence of the 40 kDa subunit demonstrated 84.4% and 67.6% homology with human and mouse sequences, respectively. The deduced sequence of the 35 kDa subunit exhibited comparable similarities to the human 35 kDa subunit (82.2%) but differed significantly (58.6%) from mouse-derived sequences.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0006-3002
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:day |
24
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pubmed:volume |
1270
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pubmed:geneSymbol |
BoIL1235,
BoIL1240
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
215-7
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pubmed:dateRevised |
2003-11-14
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pubmed:meshHeading |
pubmed-meshheading:7727547-Amino Acid Sequence,
pubmed-meshheading:7727547-Animals,
pubmed-meshheading:7727547-Base Sequence,
pubmed-meshheading:7727547-Cattle,
pubmed-meshheading:7727547-Cloning, Molecular,
pubmed-meshheading:7727547-DNA, Complementary,
pubmed-meshheading:7727547-DNA Primers,
pubmed-meshheading:7727547-Gene Amplification,
pubmed-meshheading:7727547-Interleukin-12,
pubmed-meshheading:7727547-Molecular Sequence Data,
pubmed-meshheading:7727547-Molecular Weight,
pubmed-meshheading:7727547-Polymerase Chain Reaction,
pubmed-meshheading:7727547-Protein Conformation
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pubmed:year |
1995
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pubmed:articleTitle |
Enzymatic amplification and molecular cloning of cDNA encoding the small and large subunits of bovine interleukin 12.
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pubmed:affiliation |
US Department of Agriculture, Agricultural Research Service, Parasite Immunobiology Lab, Beltsville, MD 20705, USA.
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pubmed:publicationType |
Journal Article
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