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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
1995-5-30
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pubmed:databankReference |
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pubmed:abstractText |
A gene encoding beta-mannanase from Bacillus subtilis NM-39 was cloned into Escherichia coli DH5 alpha by using pUC 18 and its nucleotide sequence was determined. The beta-mannanase gene was 1080 base pairs long and encoded a mature protein of 336 amino acids and a signal peptide of 24 amino acids. The deduced amino acid sequence of the cloned mannanase showed sequence homology with mannanase from alkalophilic Bacillus sp. strain AM-001 (about 50%).
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0006-3002
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
13
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pubmed:volume |
1243
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
552-4
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:7727534-Amino Acid Sequence,
pubmed-meshheading:7727534-Bacillus subtilis,
pubmed-meshheading:7727534-Base Sequence,
pubmed-meshheading:7727534-Cloning, Molecular,
pubmed-meshheading:7727534-Escherichia coli,
pubmed-meshheading:7727534-Mannosidases,
pubmed-meshheading:7727534-Molecular Sequence Data,
pubmed-meshheading:7727534-Protein Sorting Signals,
pubmed-meshheading:7727534-Repetitive Sequences, Nucleic Acid,
pubmed-meshheading:7727534-Restriction Mapping,
pubmed-meshheading:7727534-Sequence Analysis, DNA,
pubmed-meshheading:7727534-beta-Mannosidase
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pubmed:year |
1995
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pubmed:articleTitle |
Cloning and sequencing of beta-mannanase gene from Bacillus subtilis NM-39.
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pubmed:affiliation |
Industrial Technology development Institute, Department of Science and Technology, Manilla, Phillippines.
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pubmed:publicationType |
Journal Article
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