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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
8
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pubmed:dateCreated |
1995-5-25
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pubmed:abstractText |
The DNA-dependent protein kinase (DNA-PK) consists of three polypeptide components: Ku-70, Ku-80, and an approximately 350-kDa catalytic subunit (p350). The gene encoding the Ku-80 subunit is identical to the x-ray-sensitive group 5 complementing gene XRCC5. Expression of the Ku-80 cDNA rescues both DNA double-strand break (DSB) repair and V(D)J recombination in group 5 mutant cells. The involvement of Ku-80 in these processes suggests that the underlying defect in these mutant cells may be disruption of the DNA-PK holoenzyme. In this report we show that the p350 kinase subunit is deleted in cells derived from the severe combined immunodeficiency mouse and in the Chinese hamster ovary cell line V-3, both of which are defective in DSB repair and V(D)J recombination. A centromeric fragment of human chromosome 8 that complements the scid defect also restores p350 protein expression and rescues in vitro DNA-PK activity. These data suggest the scid gene may encode the p350 protein or regulate its expression and are consistent with a model whereby DNA-PK is a critical component of the DSB-repair pathway.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/7724535-1465419,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7724535-1505945,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7724535-1547941,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7724535-1695009,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7724535-1709732,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7724535-1730599,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7724535-1945839,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7724535-1996340,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7724535-2211668,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7724535-2247066,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7724535-2247067,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7724535-2466842,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7724535-2507541,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7724535-2573661,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7724535-3015926,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7724535-3511059,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7724535-3571334,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7724535-6823332,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7724535-7516471,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7724535-7836414,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7724535-7855601,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7724535-7939667,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7724535-8031790,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7724535-8041718,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7724535-8052631,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7724535-8073286,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7724535-8088837,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7724535-8090777,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7724535-8206892,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7724535-8270250,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7724535-8291090,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7724535-8463290,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7724535-8469973,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7724535-8486698,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7724535-8486707,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7724535-8497262
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
11
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pubmed:volume |
92
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
3171-4
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:7724535-Animals,
pubmed-meshheading:7724535-Antigens, Nuclear,
pubmed-meshheading:7724535-CHO Cells,
pubmed-meshheading:7724535-Cricetinae,
pubmed-meshheading:7724535-DNA Helicases,
pubmed-meshheading:7724535-DNA Repair,
pubmed-meshheading:7724535-DNA-Activated Protein Kinase,
pubmed-meshheading:7724535-DNA-Binding Proteins,
pubmed-meshheading:7724535-Fluorescent Antibody Technique,
pubmed-meshheading:7724535-Mice,
pubmed-meshheading:7724535-Mice, SCID,
pubmed-meshheading:7724535-Nuclear Proteins,
pubmed-meshheading:7724535-Protein-Serine-Threonine Kinases,
pubmed-meshheading:7724535-Recombination, Genetic,
pubmed-meshheading:7724535-Severe Combined Immunodeficiency
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pubmed:year |
1995
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pubmed:articleTitle |
Loss of the catalytic subunit of the DNA-dependent protein kinase in DNA double-strand-break-repair mutant mammalian cells.
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pubmed:affiliation |
Life Sciences Division, Los Alamos National Laboratory, NM 87545, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.
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