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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
1976-7-6
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pubmed:abstractText |
Phi80dargECBH DNA has been used to direct cell-free synthesis of argininosuccinase, the argH gene product in Escherichia coli K12. In vitro enzyme synthesis is sensitive to repression by partially purified preparations from an argR+ strain but not by corresponding preparations from an argR- strain. Using DNA-cellulose chromatography, approximately seventyfold purification of repressor has been obtained. The partially purified preparation represses argininosuccinase synthesis but has no effect on beta-galactosidase synthesis.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0026-8925
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
27
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pubmed:volume |
144
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
17-20
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:772411-Arginine,
pubmed-meshheading:772411-Argininosuccinate Lyase,
pubmed-meshheading:772411-Cell-Free System,
pubmed-meshheading:772411-Chromatography, DEAE-Cellulose,
pubmed-meshheading:772411-DNA,
pubmed-meshheading:772411-Enzyme Repression,
pubmed-meshheading:772411-Escherichia coli,
pubmed-meshheading:772411-Galactosidases,
pubmed-meshheading:772411-Genes,
pubmed-meshheading:772411-Lyases,
pubmed-meshheading:772411-Mutation
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pubmed:year |
1976
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pubmed:articleTitle |
In vitro synthesis and repression of argininosuccinase in Escherichia coli K12; partial purification of the arginine repressor.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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