Linked Life Data

7723777

Source:http://linkedlifedata.com/resource/pubmed/id/7723777

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1995-5-19
pubmed:abstractText
Genetic exchange can take place between different strains of Trypanosoma brucei ssp. when they are cotransmitted via the tsetse fly vector, but the mechanism and limits of compatibility between strains are ill-defined as yet. Following the recovery of several hybrid genotypes with single drug resistance from a cross of drug resistant parental strains, we attempted a series of backcrosses and F1 crosses, selecting hybrids by double drug resistance. Of 4 backcrosses, one produced hybrid progeny, the analysis of which is presented here, but none of the 4 F1 crosses produced hybrid progeny. However, among experimental flies from the 8 crosses, although there were large numbers of salivary gland infections, very few consisted of a mixture of parental clones, a prerequisite for mating. In the successful backcross both parents were diploid, but none of the crosses involving triploid clones produced hybrid progeny. The hybrid-secreting fly from the backcross contained a mixture of hybrid and parental clones. The hybrid clones had approximately 3n DNA contents relative to the 2n parental clones and fell into 2 groups with respect to restriction site polymorphisms in kinetoplast DNA maxi-circles. Fingerprinting by random PCR amplification using 8 different arbitrary primers showed minor variation between the hybrid clones.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0166-6851
pubmed:author
pubmed:issnType
Print
pubmed:volume
69
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
101-110
pubmed:dateRevised
2010-8-25
pubmed:meshHeading
pubmed:year
1995
pubmed:articleTitle
A successful backcross in Trypanosoma brucei.
pubmed:affiliation
Department of Pathology and Microbiology, University of Bristol Veterinary School, Langford, UK.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't