Linked Life Data

7722622

Source:http://linkedlifedata.com/resource/pubmed/id/7722622

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
1995-5-25
pubmed:abstractText
The present study investigated the effect of intraperitoneal (i.p.) administration of endotoxin lipopolysaccharide (LPS) and immobilization stress on the genetic expression of corticotropin-releasing factor receptor (CRF-R) in the brains of conscious male Sprague-Dawley rats. One group of rats was killed at 1, 3, 6, 9, and 12 hr after a single intraperitoneal injection of either the LPS (250 micrograms/100 gm of body weight) or the vehicle solution; the other group was killed before, immediately after, 1.5, 3, 6, and 12 hr after a 90 min acute session of immobilization stress. Rats were deeply anesthetized and rapidly perfused with a solution of 4% paraformaldehyde-borax. Frozen brains were mounted on a microtome and cut from the olfactory bulb to the medulla in 30 microns coronal sections. mRNA encoding the rat CRF-R was assayed by in situ hybridization histochemistry using a 35S-labeled riboprobe, and CRF-R localization within CRF-immunoreactive neurons in the PVN was determined using a combination of immunocytochemistry and in situ hybridization techniques. Strong basal levels of CRF-R transcripts were observed in several regions of the brain (piriform cortex, medial and basolateral nuclei of the amygdala, red nucleus, pontine gray, cerebellum, laterodorsal tegmental nucleus, caudal division of the zona incerta, nucleus incertus, spinal and principal sensory nuclei of the trigeminal nerve, and various layers of the cortex). A low to moderate signal was also detected in multiple sites (medial septal nucleus, nucleus of the diagonal band, supraoptic nucleus, arcuate nucleus of the hypothalamus, interpeduncular nucleus, and nucleus prepositus). Whereas vehicle-treated and control rats displayed hardly detectable signals of CRF-R mRNA in the paraventricular nucleus (PVN), CRF-R gene transcription was highly stimulated by LPS administration and immobilization stress in this hypothalamic structure. Indeed, the CRF-R mRNA signal was positive in the dorsomedial parvocellular PVN 3 hr after LPS injection, strong and maximum in both parvo- and magno-PVN at 6 hr postinjection, and declined 9 and 12 hr after treatment. Similarly, 90 min and 3 hr after the immobilization session, mRNA encoding the CRF-R was highly expressed in the parvo-PVN and totally vanished 12 hr after the stress. A lower but significant increase in the CRF-R transcript signal was also observed in the supraoptic nucleus 6 hr after the LPS treatment.(ABSTRACT TRUNCATED AT 400 WORDS)
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0270-6474
pubmed:author
pubmed:issnType
Print
pubmed:volume
15
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2680-95
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:7722622-Analysis of Variance, pubmed-meshheading:7722622-Animals, pubmed-meshheading:7722622-Arcuate Nucleus, pubmed-meshheading:7722622-Brain, pubmed-meshheading:7722622-Gene Expression, pubmed-meshheading:7722622-Hypothalamus, pubmed-meshheading:7722622-Immunohistochemistry, pubmed-meshheading:7722622-In Situ Hybridization, pubmed-meshheading:7722622-Lipopolysaccharides, pubmed-meshheading:7722622-Male, pubmed-meshheading:7722622-Paraventricular Hypothalamic Nucleus, pubmed-meshheading:7722622-RNA, Messenger, pubmed-meshheading:7722622-RNA Probes, pubmed-meshheading:7722622-Rats, pubmed-meshheading:7722622-Rats, Sprague-Dawley, pubmed-meshheading:7722622-Receptors, Corticotropin-Releasing Hormone, pubmed-meshheading:7722622-Restraint, Physical, pubmed-meshheading:7722622-Stress, Psychological, pubmed-meshheading:7722622-Sulfur Radioisotopes, pubmed-meshheading:7722622-Supraoptic Nucleus, pubmed-meshheading:7722622-Time Factors
pubmed:year
1995
pubmed:articleTitle
Immune challenge and immobilization stress induce transcription of the gene encoding the CRF receptor in selective nuclei of the rat hypothalamus.
pubmed:affiliation
Laboratory of Molecular Endocrinology, CHUL Research Center, Québec, Canada.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't