Linked Life Data

7722501

Source:http://linkedlifedata.com/resource/pubmed/id/7722501

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
1995-5-23
pubmed:abstractText
CBL/57 strain db/db mice exhibit type II (noninsulin-dependent) diabetes. The affected mice are markedly hyperinsulinemic, hyperglycemic, and hypercholesterolemic, and their serum K+ levels are decreased. The brains of the diabetic mice are significantly smaller than those of their lean, control littermates, but the protein concentration is normal. The low brain weight is accompanied by a loss of major fatty acid components within the whole brain, nerve endings, and mitochondrial membranes. Cholesterol levels are low in whole brain but are not significantly different from normal in the synaptosomal membranes. The phospholipid concentration is significantly decreased in whole brain homogenates, crude synaptosomal membranes, and crude mitochondrial membranes of the diabetic mice. In addition, the specific activities of membrane-bound synaptosomal acetylcholinesterase, Na+,K(+)-ATPase, and Mg(2+)-ATPase are decreased in crude synaptosomal membranes of the diabetic mice. The specific activities of carnitine palmitoyltransferase I and carnitine acetyltransferase are significantly increased in the crude mitochondrial fraction isolated from the brains of the type II diabetic mice, whereas the specific activity of pyruvate dehydrogenase complex is decreased. The specific activities of two other mitochondrial enzymes--monoamine oxidase B and citrate synthase--and a cytosolic enzyme--lactate dehydrogenase--are unaltered. The ability to synthesize cyclic AMP is markedly decreased in the brains of the diabetic mice. The concentrations of carnitine and of the amino acids, glutamate, aspartate, glutamine, and serine are unaltered, whereas glycine levels are significantly elevated in the brains of the db/db mice. The data suggest that in vivo the brains of the diabetic mice exhibit a decreased capacity for glucose oxidation and increased capacity for fatty acid oxidation. This hypothesis is supported by the finding that cerebral mitochondria isolated from the db/db mice oxidize [1-14C]palmitate to 14CO2 at a rate almost twice that of control mitochondria. The present findings emphasize the potentially serious alteration of brain metabolism in uncontrolled type II diabetes.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0022-3042
pubmed:author
pubmed:issnType
Print
pubmed:volume
64
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2159-68
pubmed:dateRevised
2007-11-15
pubmed:meshHeading
pubmed-meshheading:7722501-Acetylcholinesterase, pubmed-meshheading:7722501-Amino Acids, pubmed-meshheading:7722501-Animals, pubmed-meshheading:7722501-Brain, pubmed-meshheading:7722501-Ca(2+) Mg(2+)-ATPase, pubmed-meshheading:7722501-Carnitine O-Acetyltransferase, pubmed-meshheading:7722501-Carnitine O-Palmitoyltransferase, pubmed-meshheading:7722501-Cell Membrane, pubmed-meshheading:7722501-Diabetes Mellitus, Experimental, pubmed-meshheading:7722501-Diabetes Mellitus, Type 2, pubmed-meshheading:7722501-Fatty Acids, pubmed-meshheading:7722501-Intracellular Membranes, pubmed-meshheading:7722501-Lipid Metabolism, pubmed-meshheading:7722501-Male, pubmed-meshheading:7722501-Mice, pubmed-meshheading:7722501-Mice, Obese, pubmed-meshheading:7722501-Mitochondria, pubmed-meshheading:7722501-Pyruvate Dehydrogenase Complex, pubmed-meshheading:7722501-Sodium-Potassium-Exchanging ATPase, pubmed-meshheading:7722501-Synaptic Membranes
pubmed:year
1995
pubmed:articleTitle
Lipid metabolism and membrane composition are altered in the brains of type II diabetic mice.
pubmed:affiliation
Department of Biochemistry, Cornell University Medical College, New York, NY 10021, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.