Linked Life Data

7722425

Source:http://linkedlifedata.com/resource/pubmed/id/7722425

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
1995-5-25
pubmed:abstractText
The hypothesis that disparate neutrophil functional responses to various chemoattractants are regulated by receptor-specific rates of G protein activation was examined in HL-60 granulocytes. The initial rates of G protein activation and the affinity of receptor-stimulated G proteins for GTP gamma S in HL-60 membranes stimulated by fMet-Leu-Phe, C5a, and leukotriene B4 (LTB4) differed significantly among the chemoattractants, with a rank order of fMet-Leu-Phe > C5a > LTB4. Equilibrium GTP gamma S binding showed that all three chemoattractants activated a common pool of G proteins. Stimulation of phospholipase D activation, measured as phosphatidylethanol generation, and superoxide release in intact cells also occurred with a rank order of fMet-Leu-Phe > C5a > LTB4. On the other hand, the rank order of receptor affinities for ligand and of the EC50 of chemoattractant stimulation of GTP gamma S binding was C5a > LTB4 > fMet-Leu-Phe. C5a and LTB4 receptor densities were similar but were less than formyl peptide receptor density. Graded pertussis toxin treatment proportionally reduced superoxide release and phospholipase D activation to all three chemoattractants. The results suggest that receptor-specific differences in G protein affinity for guanine nucleotides lead to different rates of guanine nucleotide exchange and, thereby, contribute to disparate effector enzyme and functional responses.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0741-5400
pubmed:author
pubmed:issnType
Print
pubmed:volume
57
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
679-86
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:7722425-Amino Acid Sequence, pubmed-meshheading:7722425-Chemotactic Factors, pubmed-meshheading:7722425-Enzyme Activation, pubmed-meshheading:7722425-GTP-Binding Proteins, pubmed-meshheading:7722425-Granulocytes, pubmed-meshheading:7722425-Guanosine 5'-O-(3-Thiotriphosphate), pubmed-meshheading:7722425-Humans, pubmed-meshheading:7722425-Kinetics, pubmed-meshheading:7722425-Leukemia, Myeloid, pubmed-meshheading:7722425-Molecular Sequence Data, pubmed-meshheading:7722425-N-Formylmethionine Leucyl-Phenylalanine, pubmed-meshheading:7722425-Phospholipase D, pubmed-meshheading:7722425-Receptors, Formyl Peptide, pubmed-meshheading:7722425-Receptors, Immunologic, pubmed-meshheading:7722425-Receptors, Peptide, pubmed-meshheading:7722425-Stimulation, Chemical, pubmed-meshheading:7722425-Substrate Specificity
pubmed:year
1995
pubmed:articleTitle
Chemoattractant receptor-specific differences in G protein activation rates regulate effector enzyme and functional responses.
pubmed:affiliation
Department of Medicine, University of Louisville, KY 40292, USA.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, U.S. Gov't, Non-P.H.S., Research Support, Non-U.S. Gov't