Linked Life Data

772162

Source:http://linkedlifedata.com/resource/pubmed/id/772162

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1976-7-6
pubmed:abstractText
The extent of the deletions of five Tets Tra- mutants of R100-1 was determined by complementation experiments with wild-type and tra mutants of Flac. The presence or absence of the origin of transfer on the mutants was also investigated. Using the results, a tentative map of this region of the R factor was drawn: it was essentially similar to the analogous region of the E. coli K12 F factor, except that tet was located between traJ and traA. Some of the deletions had removed the promoter for the transfer operon. This allowed detection of the transcription of traC and distal genes from a weak, traJ-independent promoter. This is probably the Is2 promoter, since R100-1 carries and Is2 insertion sequence located immediately to the left of traC in the correct orientation. Since neither the transfer operon promoter nor the Is2 promoter seemed to be required for transcription of traI, it was concluded that, unlike the F factor, this was located in a separate operon.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0022-1287
pubmed:author
pubmed:issnType
Print
pubmed:volume
93
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
133-40
pubmed:dateRevised
2006-4-13
pubmed:meshHeading
pubmed:year
1976
pubmed:articleTitle
Genetic analysis of deletions of R100-1 that are both transfer-deficient and tetracycline-sensitive.
pubmed:publicationType
Journal Article