Linked Life Data

7720703

Source:http://linkedlifedata.com/resource/pubmed/id/7720703

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
1995-5-22
pubmed:abstractText
The peripheral myelin gene PMP22 is the rat and human homologue of the murine growth arrest-specific gene gas3. Besides a putative role of PMP22 in myelination, a regulatory function in cell growth has been suspected. Here we have investigated both the expression of PMP22 during cell cycle progression of cultured rat Schwann cells and the influence of altered levels of PMP22 on Schwann cell growth. When resting cells were stimulated to begin the cell cycle, the regulation of PMP22 mRNA resembled the growth arrest-specific pattern of gas3 expression observed previously in NIH3T3 fibroblasts. To prove a growth regulatory function of PMP22, we generated Schwann cell cultures by infection with retroviral PMP22 expression vectors that constitutively expressed PMP22 cDNA sequences, in either the sense or antisense orientation. Transduced cells carrying the sense construct overexpressed PMP22 mRNA and protein, whereas in cells infected with an antisense PMP22 expression vector PMP22 mRNA levels were reduced markedly. Altered levels of PMP22 significantly modulated Schwann cell proliferation, as judged by 5-bromo-2'-deoxy-uridine incorporation into replicated DNA. In asynchronously dividing cultures enhanced expression of PMP22 decreased DNA synthesis to 60% of the control level. Conversely, reduced levels of PMP22 mRNA led to enhanced DNA synthesis of approximately 150%. Further cell cycle analyses by flow cytometry revealed that overexpression of PMP22 delayed serum- and forskolin-stimulated entry of resting Schwann cells from G0/G1 into the S + G2/M phases by approximately 8 h, whereas underexpression of PMP22 mRNA slightly increased the proportion of cells that entered the S + G2/M phases.(ABSTRACT TRUNCATED AT 250 WORDS)
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/7720703-1303228, http://linkedlifedata.com/resource/pubmed/commentcorrection/7720703-1303229, http://linkedlifedata.com/resource/pubmed/commentcorrection/7720703-1303231, http://linkedlifedata.com/resource/pubmed/commentcorrection/7720703-1303281, http://linkedlifedata.com/resource/pubmed/commentcorrection/7720703-1374899, http://linkedlifedata.com/resource/pubmed/commentcorrection/7720703-1505026, http://linkedlifedata.com/resource/pubmed/commentcorrection/7720703-1552943, http://linkedlifedata.com/resource/pubmed/commentcorrection/7720703-1556154, http://linkedlifedata.com/resource/pubmed/commentcorrection/7720703-1607387, http://linkedlifedata.com/resource/pubmed/commentcorrection/7720703-1630459, http://linkedlifedata.com/resource/pubmed/commentcorrection/7720703-1692961, http://linkedlifedata.com/resource/pubmed/commentcorrection/7720703-1714591, http://linkedlifedata.com/resource/pubmed/commentcorrection/7720703-1724420, http://linkedlifedata.com/resource/pubmed/commentcorrection/7720703-1935894, http://linkedlifedata.com/resource/pubmed/commentcorrection/7720703-2181447, http://linkedlifedata.com/resource/pubmed/commentcorrection/7720703-2440339, http://linkedlifedata.com/resource/pubmed/commentcorrection/7720703-2683075, http://linkedlifedata.com/resource/pubmed/commentcorrection/7720703-3193129, http://linkedlifedata.com/resource/pubmed/commentcorrection/7720703-3409319, http://linkedlifedata.com/resource/pubmed/commentcorrection/7720703-371755, http://linkedlifedata.com/resource/pubmed/commentcorrection/7720703-4345910, http://linkedlifedata.com/resource/pubmed/commentcorrection/7720703-6313869, http://linkedlifedata.com/resource/pubmed/commentcorrection/7720703-6331674, http://linkedlifedata.com/resource/pubmed/commentcorrection/7720703-6530509, http://linkedlifedata.com/resource/pubmed/commentcorrection/7720703-6678608, http://linkedlifedata.com/resource/pubmed/commentcorrection/7720703-7231639, http://linkedlifedata.com/resource/pubmed/commentcorrection/7720703-7504284, http://linkedlifedata.com/resource/pubmed/commentcorrection/7720703-7512319, http://linkedlifedata.com/resource/pubmed/commentcorrection/7720703-7691737, http://linkedlifedata.com/resource/pubmed/commentcorrection/7720703-8021974, http://linkedlifedata.com/resource/pubmed/commentcorrection/7720703-8028042, http://linkedlifedata.com/resource/pubmed/commentcorrection/7720703-8162049, http://linkedlifedata.com/resource/pubmed/commentcorrection/7720703-8252046, http://linkedlifedata.com/resource/pubmed/commentcorrection/7720703-8275092, http://linkedlifedata.com/resource/pubmed/commentcorrection/7720703-8316854, http://linkedlifedata.com/resource/pubmed/commentcorrection/7720703-8336730, http://linkedlifedata.com/resource/pubmed/commentcorrection/7720703-8357825, http://linkedlifedata.com/resource/pubmed/commentcorrection/7720703-8422677, http://linkedlifedata.com/resource/pubmed/commentcorrection/7720703-8486695, http://linkedlifedata.com/resource/pubmed/commentcorrection/7720703-8510709, http://linkedlifedata.com/resource/pubmed/commentcorrection/7720703-999941
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0261-4189
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
14
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1122-8
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1995
pubmed:articleTitle
Retroviral-mediated gene transfer of the peripheral myelin protein PMP22 in Schwann cells: modulation of cell growth.
pubmed:affiliation
Department of Neurology, Heinrich-Heine-University of Düsseldorf, Germany.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't