Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
1995-5-22
pubmed:databankReference
pubmed:abstractText
A member of a new subclass of the voltage-activated sodium channel genes has been cloned from the human medullary thyroid carcinoma (hMTC) cell line. The cDNA of hNE-Na (human neuroendocrine sodium channel) encodes a 1977 amino acid protein which phylogenetically represents a link between sodium channels isolated from skeletal muscle and brain. The hNE-Na alpha subunit was transiently expressed in human embryonic kidney cells either alone or in combination with the human sodium channel beta 1 subunit. The channel exhibited rapid activation and inactivation kinetics, and was blocked by tetrodotoxin and cadmium with IC50 values of 24.5 nM and 1.1 mM, respectively. Action potentials were generated in cells expressing high levels of hNE-Na. Northern blot and reverse transcription-polymerase chain reaction (RT-PCR) analyses demonstrated its expression in hMTC cells, in a C-cell carcinoma, and in thyroid and adrenal gland. Transcripts were not identified in pituitary gland, brain, heart, liver or kidney, indicating that the hNE-Na is a sodium channel solely expressed in neuroendocrine cells.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/7720699-1034699, http://linkedlifedata.com/resource/pubmed/commentcorrection/7720699-1309650, http://linkedlifedata.com/resource/pubmed/commentcorrection/7720699-1309946, http://linkedlifedata.com/resource/pubmed/commentcorrection/7720699-1313507, http://linkedlifedata.com/resource/pubmed/commentcorrection/7720699-1317577, http://linkedlifedata.com/resource/pubmed/commentcorrection/7720699-1332059, http://linkedlifedata.com/resource/pubmed/commentcorrection/7720699-1375395, http://linkedlifedata.com/resource/pubmed/commentcorrection/7720699-1375397, http://linkedlifedata.com/resource/pubmed/commentcorrection/7720699-1379737, http://linkedlifedata.com/resource/pubmed/commentcorrection/7720699-1443107, http://linkedlifedata.com/resource/pubmed/commentcorrection/7720699-1591615, http://linkedlifedata.com/resource/pubmed/commentcorrection/7720699-2159418, http://linkedlifedata.com/resource/pubmed/commentcorrection/7720699-2449363, http://linkedlifedata.com/resource/pubmed/commentcorrection/7720699-2554301, http://linkedlifedata.com/resource/pubmed/commentcorrection/7720699-2555170, http://linkedlifedata.com/resource/pubmed/commentcorrection/7720699-2557243, http://linkedlifedata.com/resource/pubmed/commentcorrection/7720699-3754035, http://linkedlifedata.com/resource/pubmed/commentcorrection/7720699-6296372, http://linkedlifedata.com/resource/pubmed/commentcorrection/7720699-7516685, http://linkedlifedata.com/resource/pubmed/commentcorrection/7720699-7518938, http://linkedlifedata.com/resource/pubmed/commentcorrection/7720699-7680747, http://linkedlifedata.com/resource/pubmed/commentcorrection/7720699-8179845, http://linkedlifedata.com/resource/pubmed/commentcorrection/7720699-8384790, http://linkedlifedata.com/resource/pubmed/commentcorrection/7720699-8394370, http://linkedlifedata.com/resource/pubmed/commentcorrection/7720699-8394762
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0261-4189
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
14
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1084-90
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed-meshheading:7720699-Amino Acid Sequence, pubmed-meshheading:7720699-Animals, pubmed-meshheading:7720699-Base Sequence, pubmed-meshheading:7720699-Carcinoma, Medullary, pubmed-meshheading:7720699-Cloning, Molecular, pubmed-meshheading:7720699-Humans, pubmed-meshheading:7720699-Membrane Potentials, pubmed-meshheading:7720699-Molecular Sequence Data, pubmed-meshheading:7720699-Organ Specificity, pubmed-meshheading:7720699-Phylogeny, pubmed-meshheading:7720699-RNA, Messenger, pubmed-meshheading:7720699-Rats, pubmed-meshheading:7720699-Sequence Analysis, DNA, pubmed-meshheading:7720699-Sequence Homology, Amino Acid, pubmed-meshheading:7720699-Sodium Channels, pubmed-meshheading:7720699-Species Specificity, pubmed-meshheading:7720699-Tetrodotoxin, pubmed-meshheading:7720699-Thyroid Gland, pubmed-meshheading:7720699-Thyroid Neoplasms, pubmed-meshheading:7720699-Tumor Cells, Cultured
pubmed:year
1995
pubmed:articleTitle
Structure and functional expression of a new member of the tetrodotoxin-sensitive voltage-activated sodium channel family from human neuroendocrine cells.
pubmed:affiliation
Institut für Pharmakologie und Toxikologie Technischen Universität München, Germany.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't