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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
5
|
| pubmed:dateCreated |
1995-5-23
|
| pubmed:abstractText |
We demonstrate that androgens rapidly and specifically increase intracellular calcium in Sertoli cells, investigate the mechanism, and suggest the unifying hypothesis that calcium might be a common intracellular molecular effector to explain the known synergism between FSH and testosterone (T) action on Sertoli cells in support of spermatogenesis. In freshly isolated Sertoli cells, T and its 5 alpha-reduced metabolite dihydrotestosterone increased intracellular calcium from 83 +/- 4 to 147 +/- 8 and 167 +/- 29 nM, respectively, whereas estradiol had minor (117 +/- 9 nM) and progesterone no (80 +/- 6 nM) effect. The effect of T was rapid (20-40 sec) and inhibited by 1) preincubation with either a pure nonsteroidal antiandrogen (hydroxyflutamide) or a 5 alpha-reductase inhibitor (finasteride) or 2) removal of extracellular calcium (47 +/- 4 nM) or pharmacological blockade of voltage-activated (62 +/- 5 nM) or voltage-independent (55 +/- 14 nM) membrane calcium channels. These findings suggest that the T-induced rise in Sertoli cell cytosolic calcium involves sequential 5 alpha-reduction, binding to a classical androgen receptor, and activation of transmembrane influx of extracellular calcium. Immobilization of T by conjugation to a large carrier molecule (BSA) to prevent steroid entry into Sertoli cells also resulted in a rapid increase in cytosolic calcium to a similar magnitude as unconjugated T, consistent with a plasma membrane site of action. This finding together with the rapid cytosolic calcium rise caused by T argues for the possible existence of a short term, nongenomic effects in hormonal regulation of Sertoli cell function in addition to the well known, slower genomic response.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
AIM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Androgen Antagonists,
http://linkedlifedata.com/resource/pubmed/chemical/Androgens,
http://linkedlifedata.com/resource/pubmed/chemical/Calcium,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclic AMP,
http://linkedlifedata.com/resource/pubmed/chemical/Dihydrotestosterone,
http://linkedlifedata.com/resource/pubmed/chemical/Finasteride,
http://linkedlifedata.com/resource/pubmed/chemical/Flutamide,
http://linkedlifedata.com/resource/pubmed/chemical/Follicle Stimulating Hormone,
http://linkedlifedata.com/resource/pubmed/chemical/Progesterone,
http://linkedlifedata.com/resource/pubmed/chemical/Testosterone,
http://linkedlifedata.com/resource/pubmed/chemical/hydroxyflutamide
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
May
|
| pubmed:issn |
0013-7227
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
136
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
2052-9
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:7720654-Androgen Antagonists,
pubmed-meshheading:7720654-Androgens,
pubmed-meshheading:7720654-Animals,
pubmed-meshheading:7720654-Calcium,
pubmed-meshheading:7720654-Cells, Cultured,
pubmed-meshheading:7720654-Cyclic AMP,
pubmed-meshheading:7720654-Cytosol,
pubmed-meshheading:7720654-Dihydrotestosterone,
pubmed-meshheading:7720654-Drug Synergism,
pubmed-meshheading:7720654-Finasteride,
pubmed-meshheading:7720654-Flutamide,
pubmed-meshheading:7720654-Follicle Stimulating Hormone,
pubmed-meshheading:7720654-Kinetics,
pubmed-meshheading:7720654-Male,
pubmed-meshheading:7720654-Progesterone,
pubmed-meshheading:7720654-Rats,
pubmed-meshheading:7720654-Rats, Wistar,
pubmed-meshheading:7720654-Sertoli Cells,
pubmed-meshheading:7720654-Spermatogenesis,
pubmed-meshheading:7720654-Testosterone,
pubmed-meshheading:7720654-Time Factors
|
| pubmed:year |
1995
|
| pubmed:articleTitle |
Androgens rapidly increase the cytosolic calcium concentration in Sertoli cells.
|
| pubmed:affiliation |
Department of Obstetrics and Gynecology, University of Sydney, New South Wales, Australia.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|