Linked Life Data

7720218

Source:http://linkedlifedata.com/resource/pubmed/id/7720218

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1995-5-25
pubmed:abstractText
Peripheral-type benzodiazepine receptors (PBRs) in brain are primarily localized within astroglial cells, and the existence of PBR subtypes have been suggested. In the present study the ontogeny of PBRs labeled with [3H]Ro5-4864 and [3H]PK 11195 in cultured astrocytes was compared to their postnatal in-vivo development. The density of [3H]Ro5-4864 binding sites in cultured astrocytes from rat cortex progressively increased from 1- to 3-week-old cultures and did not change further in 5- and 8-week-old cultures. The density of [3H]PK 11195 binding sites progressively increased from 1- to 5-week-old cultures. The density of [3H]PK 11195 binding sites exceeded the density of [3H]Ro5-4864 binding sites by 40-50% in 2-, 5- and 8-week-old cultures. The affinity of the PBR ligands for the receptor sites was increased by 3- to 4-fold from the first to the second week in cultures, and did not change thereafter. A similar developmental pattern of PBRs was observed in rat cortex, except that: first, the difference between the Bmax of [3H]PK 11195 and [3H]Ro5-4864 was already apparent in postnatal-week-1 and persisted with maturation; second, the high affinity of the ligands for the receptor sites was apparent from postnatal-week-1 and did not change with maturation. Age-related differences in the ratio between the density of PBRs in astrocytes and rat cortex were also observed. These results lead us to suggest that the development of PBRs in vivo during the first postnatal week is more rapid than the development of the receptors in vitro during the first week in culture. Subsequently, the increased ratio between the density of PBRs in cultured astrocytes and brain with maturation indicates the predominantly astrocytic localization of these receptors. The finding that the density of [3H]PK 11195 binding sites in cultured astrocytes and in rat brain cortex is usually 40-50% greater than the density of [3H]Ro5-4864 binding sites further supports the existence of PBR subtypes.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0165-3806
pubmed:author
pubmed:issnType
Print
pubmed:day
14
pubmed:volume
84
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
62-6
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1995
pubmed:articleTitle
Ontogeny of peripheral-type benzodiazepine receptors in cultured astrocytes and brain from rat.
pubmed:affiliation
Department of Biochemistry and Molecular Biology, University of Miami School of Medicine, FL, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, U.S. Gov't, Non-P.H.S., Research Support, Non-U.S. Gov't