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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
1995-5-24
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pubmed:abstractText |
Three procedures for the removal of immunoglobulin G (IgG) from human serum were evaluated for their effectiveness in eliminating false-positive results caused by rheumatoid factor and in removing IgG from serum to reduce competing-IgG interference in IgM enzyme-linked immunosorbent assay (ELISA) testing. The procedures investigated employed two anti-human IgG diluents and a recombinant protein G-filled tube. The anti-human IgG was more effective than the protein G method in eliminating false-positive results caused by rheumatoid factor and removed 5.4% more IgG from serum samples in the normal range (< 1,700 mg/dl) and up to 16.4% more of the IgG from samples with elevated levels (> 1,700 mg/dl). The recombinant protein G removed less IgM than the anti-human IgG diluents; however, this difference did not affect the results of the ELISA. For these reasons, the in-house-developed anti-human IgG diluent proved to be the most effective and economical for IgM serological testing.
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pubmed:commentsCorrections |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Anti-Idiotypic,
http://linkedlifedata.com/resource/pubmed/chemical/Antigen-Antibody Complex,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Viral,
http://linkedlifedata.com/resource/pubmed/chemical/Capsid Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Epstein-Barr viral capsid antigen,
http://linkedlifedata.com/resource/pubmed/chemical/G-substrate,
http://linkedlifedata.com/resource/pubmed/chemical/Immunoglobulin G,
http://linkedlifedata.com/resource/pubmed/chemical/Immunoglobulin M,
http://linkedlifedata.com/resource/pubmed/chemical/Nerve Tissue Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Rheumatoid Factor,
http://linkedlifedata.com/resource/pubmed/chemical/Staphylococcal Protein A
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
1071-412X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
2
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
98-103
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:7719920-Animals,
pubmed-meshheading:7719920-Antibodies, Anti-Idiotypic,
pubmed-meshheading:7719920-Antigen-Antibody Complex,
pubmed-meshheading:7719920-Antigens, Viral,
pubmed-meshheading:7719920-Capsid Proteins,
pubmed-meshheading:7719920-Centrifugation,
pubmed-meshheading:7719920-Enzyme-Linked Immunosorbent Assay,
pubmed-meshheading:7719920-Evaluation Studies as Topic,
pubmed-meshheading:7719920-False Negative Reactions,
pubmed-meshheading:7719920-False Positive Reactions,
pubmed-meshheading:7719920-Goats,
pubmed-meshheading:7719920-Humans,
pubmed-meshheading:7719920-Immunoglobulin G,
pubmed-meshheading:7719920-Immunoglobulin M,
pubmed-meshheading:7719920-Immunosorbent Techniques,
pubmed-meshheading:7719920-Nephelometry and Turbidimetry,
pubmed-meshheading:7719920-Nerve Tissue Proteins,
pubmed-meshheading:7719920-Recombinant Proteins,
pubmed-meshheading:7719920-Reproducibility of Results,
pubmed-meshheading:7719920-Rheumatoid Factor,
pubmed-meshheading:7719920-Staphylococcal Protein A
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pubmed:year |
1995
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pubmed:articleTitle |
An evaluation of the effectiveness of three immunoglobulin G (IgG) removal procedures for routine IgM serological testing.
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pubmed:affiliation |
Department of Immunology, Associated Regional and University Pathologists, Salt Lake City, UT 84108.
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pubmed:publicationType |
Journal Article
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