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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
8
|
| pubmed:dateCreated |
1995-5-25
|
| pubmed:abstractText |
Interleukin-12 (IL-12), a cytokine with in vitro and in vivo immunomodulatory effects, is produced by lymphocytes and stimulated monocytes. Little is known about the production and possible role of IL-12 in human lymphoproliferative disorders. We examined IL-12 expression by immunohistochemistry using antibodies recognizing the p40, p35 subunits, and the p70 heterodimeric IL-12 protein, and by Northern blot in lymph nodes from patients with Hodgkin's disease (HD), non-Hodgkin's lymphomas (NHL), and nonneoplastic lymphoid lesions. In the majority of the HD cases (28 of 34), IL-12 immunoreaction was found in small lymphoid cells cultured around Hodgkin and Reed-Sternberg (H&RS) cells. No IL-12 signal was seen in H&RS cells. Transcripts for IL-12 were found by Northern and dot blot analysis in 13 of 19 (IL-12 p40) and 11 of 19 (IL-12 p35) cases. The HD cases were further examined for the presence of Epstein-Barr virus (EBV) latent membrane protein (LMP-1). All cases with EBV-LMP-1 positivity (22 of 34 cases) also expressed IL-12. No IL-12 immunoreaction was found in neoplastic cells of 33 cases of various NHLs, which were all LMP-1 negative and showed no EBV-genome sequence, as assessed by polymerase chain reaction (PCR). In 24 nonneoplastic lymphoid lesions, few dispersed IL-12 positive cells were seen in the parafollicular area and in the sinus of the lymph node. The marked presence of IL-12 in the majority of HD cases indicates that IL-12 might play a role in the pathobiology of HD, suggesting that this cytokine is involved in EBV-positive HD.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
AIM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Biological Markers,
http://linkedlifedata.com/resource/pubmed/chemical/EBV-associated membrane antigen...,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-12,
http://linkedlifedata.com/resource/pubmed/chemical/Neoplasm Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Neoplasm,
http://linkedlifedata.com/resource/pubmed/chemical/Viral Matrix Proteins
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Apr
|
| pubmed:issn |
0006-4971
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
15
|
| pubmed:volume |
85
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
2182-8
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:7718889-Biological Markers,
pubmed-meshheading:7718889-Gene Expression Regulation, Neoplastic,
pubmed-meshheading:7718889-Gene Expression Regulation, Viral,
pubmed-meshheading:7718889-Herpesviridae Infections,
pubmed-meshheading:7718889-Herpesvirus 4, Human,
pubmed-meshheading:7718889-Hodgkin Disease,
pubmed-meshheading:7718889-Humans,
pubmed-meshheading:7718889-Hyperplasia,
pubmed-meshheading:7718889-Interleukin-12,
pubmed-meshheading:7718889-Lymph Nodes,
pubmed-meshheading:7718889-Lymphadenitis,
pubmed-meshheading:7718889-Lymphoma,
pubmed-meshheading:7718889-Lymphoma, Non-Hodgkin,
pubmed-meshheading:7718889-Neoplasm Proteins,
pubmed-meshheading:7718889-RNA, Messenger,
pubmed-meshheading:7718889-RNA, Neoplasm,
pubmed-meshheading:7718889-Reed-Sternberg Cells,
pubmed-meshheading:7718889-Tumor Virus Infections,
pubmed-meshheading:7718889-Viral Matrix Proteins
|
| pubmed:year |
1995
|
| pubmed:articleTitle |
Interleukin-12 expression in human lymphomas and nonneoplastic lymphoid disorders.
|
| pubmed:affiliation |
Laboratory for Clinical and Experimental Research, Berne, Switzerland.
|
| pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
|