Linked Life Data

7712460

Source:http://linkedlifedata.com/resource/pubmed/id/7712460

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
8
pubmed:dateCreated
1995-5-15
pubmed:databankReference
pubmed:abstractText
Recently the putative tumor suppressor gene p16INK4 was mapped to human chromosome 9p21, which is homologous to rat chromosome 5. Monosomy of rat chromosome 5 occurs with high frequency in rat kidney tumor-derived cell lines (ERC lines). Thus, we studied these lines in order to investigate the involvement of p15INK4B and p16INK4 in the genesis of this tumor type. p15INK4B and p16INK4 were found by Southern blot analysis to be codeleted in five of seven of these lines. This was confirmed by Northern blot analysis with a probe for the rat p15INK4B gene. In normal rat tissues, expression of p15INK4B was abundant in lung (2.5 and 2.0 kilobases), less abundant in testis (2.5, 2.0, 1.1, and 0.9 kilobases), barely detectable in liver (2.0 kilobases), and not detectable in neonatal kidney, adult kidney, brain, heart, or spleen. In the ERC lines, p15INK4B was expressed as a single 2.0-kilobase transcript observed only in those cell lines in which the gene was detected by Southern blot analysis. However, neither p15INK4B nor p16INK4 were deleted in 12 of 12 primary kidney tumors examined, suggesting that deletion of these genes is not directly involved in the process of renal tumor development but may be related to tumor progression or autonomous growth in vitro. A panel of rat kidney epithelial cell lines chemically transformed in vitro (TRKE lines) that had high-frequency monosomy 5 were also examined, but deletion of p15INK4B and p16INK4 was observed in only one of six of the TRKE lines. To our knowledge, this is the first reported investigation of these genes in rodent tumors and cell lines, and its data support the theory that alterations of genes located in the INF region of rat chromosome 5 may play a role in rodent cell transformation.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0008-5472
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
55
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1607-12
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed-meshheading:7712460-Animals, pubmed-meshheading:7712460-Base Sequence, pubmed-meshheading:7712460-Carrier Proteins, pubmed-meshheading:7712460-Cell Cycle Proteins, pubmed-meshheading:7712460-Cell Line, pubmed-meshheading:7712460-Chromosome Deletion, pubmed-meshheading:7712460-Chromosome Mapping, pubmed-meshheading:7712460-Chromosomes, Human, Pair 9, pubmed-meshheading:7712460-Cyclin-Dependent Kinase Inhibitor p15, pubmed-meshheading:7712460-Cyclin-Dependent Kinase Inhibitor p16, pubmed-meshheading:7712460-Epithelium, pubmed-meshheading:7712460-Gene Expression, pubmed-meshheading:7712460-Genes, Tumor Suppressor, pubmed-meshheading:7712460-Humans, pubmed-meshheading:7712460-Karyotyping, pubmed-meshheading:7712460-Kidney Neoplasms, pubmed-meshheading:7712460-Liver, pubmed-meshheading:7712460-Lung, pubmed-meshheading:7712460-Male, pubmed-meshheading:7712460-Molecular Sequence Data, pubmed-meshheading:7712460-Monosomy, pubmed-meshheading:7712460-Oligonucleotide Probes, pubmed-meshheading:7712460-Organ Specificity, pubmed-meshheading:7712460-Rats, pubmed-meshheading:7712460-Sequence Homology, Nucleic Acid, pubmed-meshheading:7712460-Testis, pubmed-meshheading:7712460-Tumor Cells, Cultured, pubmed-meshheading:7712460-Tumor Suppressor Proteins
pubmed:year
1995
pubmed:articleTitle
Association of rat p15INK4B/p16INK4 deletions with monosomy 5 in kidney epithelial cell lines but not primary renal tumors.
pubmed:affiliation
University of Texas M. D. Anderson Cancer Center, Smithville 78957, USA.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, U.S. Gov't, P.H.S.