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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
4
|
| pubmed:dateCreated |
1995-5-17
|
| pubmed:abstractText |
The purpose of this study was to investigate the possible in vivo effects of (-)-nicotine, ethanol, and an adenosine agonist N6-cyclohexyladenosine (CHA) when injected individually as well as in various combinations on glucose utilization in the fresh cerebellar slices of mice. Mice received ICV (-)-nicotine or CHA followed 5 min later by a test dose of ethanol (2 g/kg; IP). Animals were killed 20 min postethanol treatment and fresh slices (300 microns) of cerebellum were incubated in a glucose medium in Warburg flasks using 14C-glucose as a tracer. Trapped 14CO2 was counted to estimate glucose utilization. Ethanol treatment markedly accentuated glucose utilization, whereas the pretreatment with (-)-nicotine (125 and 250 ng, ICV) resulted in a significant attenuation in the ethanol-induced increase in glucose utilization. However, ICV (-)-nicotine (125 ng) alone did not produce any change in the cerebellar glucose utilization. The attenuation of ethanol-induced increase in glucose utilization by (-)-nicotine was nearly totally blocked by ICV hexamethonium, a purported nicotinic antagonist, suggesting participation of cholinergic-nicotinic receptors. The (-)-nicotine pretreatment also significantly attenuated both the ICV CHA (25 ng)-induced increase in glucose utilization and the accentuation of ethanol-induced increase in glucose utilization by CHA. The antagonistic effect of (-)-nicotine on CHA- and ethanol-induced increase in glucose utilization indicating an interaction between (-)-nicotine and ethanol and between (-)-nicotine and adenosine may suggest involvement of postreceptor (nicotinic and adenosine) mechanisms including ionic channels.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Adenosine,
http://linkedlifedata.com/resource/pubmed/chemical/Ethanol,
http://linkedlifedata.com/resource/pubmed/chemical/Glucose,
http://linkedlifedata.com/resource/pubmed/chemical/Hexamethonium,
http://linkedlifedata.com/resource/pubmed/chemical/Nicotine
|
| pubmed:status |
MEDLINE
|
| pubmed:issn |
0361-9230
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
36
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
343-8
|
| pubmed:dateRevised |
2003-11-14
|
| pubmed:meshHeading |
pubmed-meshheading:7712193-Adenosine,
pubmed-meshheading:7712193-Animals,
pubmed-meshheading:7712193-Cerebellum,
pubmed-meshheading:7712193-Drug Interactions,
pubmed-meshheading:7712193-Ethanol,
pubmed-meshheading:7712193-Glucose,
pubmed-meshheading:7712193-Hexamethonium,
pubmed-meshheading:7712193-Male,
pubmed-meshheading:7712193-Mice,
pubmed-meshheading:7712193-Nicotine
|
| pubmed:year |
1995
|
| pubmed:articleTitle |
In vivo effects of (-)-nicotine on ethanol-induced increase in glucose utilization in the mouse cerebellum.
|
| pubmed:affiliation |
Department of Pharmacology, School of Medicine, East Carolina University, Greenville, NC 27858.
|
| pubmed:publicationType |
Journal Article
|