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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
1995-5-18
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pubmed:abstractText |
We have assembled a micropipette aspiration assay to measure membrane destabilization events in which large (20-30 microns diameter) unilamellar vesicles are manipulated and exposed to membrane destabilizing agents. Single events can be seen with a light microscope and are recorded using both a video camera and a photomultiplier tube. We have performed experiments with a wild-type fusion peptide from influenza virus (X31) and found that it induces pH-dependent, stochastic lysis of large unilamellar vesicles. The rate and extent of lysis are both maximum at pH 5; the maximum rate of lysis is 0.018 s-1 at pH 5. An analysis of our data indicates that the lysis is not correlated either to the size of the vesicles or to the tension created in the vesicle membranes by aspiration.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/7711257-1323686,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7711257-1439803,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7711257-1600107,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7711257-1931950,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7711257-2021611,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7711257-2047522,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7711257-2055263,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7711257-2109749,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7711257-2184772,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7711257-2265606,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7711257-2265687,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7711257-2466492,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7711257-2660823,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7711257-2720083,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7711257-2784694,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7711257-3191124,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7711257-3233209,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7711257-3338569,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7711257-3404116,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7711257-3694175,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7711257-3753607,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7711257-3967299,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7711257-4063343,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7711257-4509315,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7711257-5765779,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7711257-6202334,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7711257-6487740,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7711257-6619506,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7711257-698340,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7711257-7260275,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7711257-7272454,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7711257-7678310,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7711257-8075329,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7711257-8133242,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7711257-8293471,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7711257-8347997,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7711257-8350749,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7711257-8387488,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7711257-839528,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7711257-8466935,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7711257-8486735,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7711257-8500173
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0006-3495
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
68
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
315-25
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:7711257-Amino Acid Sequence,
pubmed-meshheading:7711257-Biophysical Phenomena,
pubmed-meshheading:7711257-Biophysics,
pubmed-meshheading:7711257-Hydrogen-Ion Concentration,
pubmed-meshheading:7711257-Liposomes,
pubmed-meshheading:7711257-Membrane Fusion,
pubmed-meshheading:7711257-Methods,
pubmed-meshheading:7711257-Molecular Sequence Data,
pubmed-meshheading:7711257-Orthomyxoviridae,
pubmed-meshheading:7711257-Spectrometry, Fluorescence,
pubmed-meshheading:7711257-Viral Fusion Proteins
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pubmed:year |
1995
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pubmed:articleTitle |
Micropipette manipulation technique for the monitoring of pH-dependent membrane lysis as induced by the fusion peptide of influenza virus.
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pubmed:affiliation |
School of Chemical Engineering, Cornell University, Ithaca, New York 14853.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't
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