|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
4
|
|
pubmed:dateCreated |
1995-5-12
|
|
pubmed:abstractText |
We have investigated the influence of interleukin 1 (IL-1) on growth of human renal carcinoma cells in vitro. Using a capillary soft-agar cloning system, 18% of freshly explanted renal carcinomas were stimulated to grow by IL-1 and 4% were inhibited. Subsequent experiments with established renal cancer cell lines demonstrated that two out of four cell lines (Caki-2, A-498) were sensitive to IL-1. [3H]Thymidine incorporation as well as monolayer growth was enhanced in Caki-2 cells in the presence of high (10%) and low (1%) serum concentrations. Although clonogenic growth of A-498 cells was stimulated by IL-1, overall [3H]thymidine incorporation and monolayer proliferation were decreased. Using radioligand experiments, 250 cell-surface receptors of high affinity (KD 4.5 x 10(-11) M) and 2500 receptors of low affinity (KD 1.3 x 10(-9) M) were detected on A-498 cells. IL-1 binding was reduced under the influence of IL-1. Competition experiments with inhibiting antibodies against IL-1 receptor type I and type II revealed that signal transduction was performed via type I receptors. After cross-linking to IL-1, receptor type I was immunoprecipitated using anti-IL-1 antibodies. We hypothesise that, since IL-1 modulates in vitro growth of a subgroup of human renal cancer cells, interference with its mechanism of action may be of potential value in order to modulate tumour proliferation.
|
|
pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/7710946-1322742,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7710946-1426100,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7710946-1533388,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7710946-1833184,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7710946-2151761,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7710946-2196116,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7710946-2293911,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7710946-2952728,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7710946-2970890,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7710946-2971135,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7710946-3110477,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7710946-3160805,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7710946-5432063,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7710946-6163158,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7710946-8327496,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7710946-8332913,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7710946-8421076,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7710946-8428929
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Apr
|
|
pubmed:issn |
0007-0920
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:volume |
71
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
794-800
|
|
pubmed:dateRevised |
2009-11-18
|
|
pubmed:meshHeading |
pubmed-meshheading:7710946-Carcinoma, Renal Cell,
pubmed-meshheading:7710946-Cell Division,
pubmed-meshheading:7710946-Cell Line,
pubmed-meshheading:7710946-Colonic Neoplasms,
pubmed-meshheading:7710946-Culture Media,
pubmed-meshheading:7710946-Humans,
pubmed-meshheading:7710946-Interleukin-1,
pubmed-meshheading:7710946-Kidney Neoplasms,
pubmed-meshheading:7710946-Kinetics,
pubmed-meshheading:7710946-Receptors, Interleukin-1,
pubmed-meshheading:7710946-Recombinant Proteins,
pubmed-meshheading:7710946-Stomach Neoplasms,
pubmed-meshheading:7710946-Thymidine,
pubmed-meshheading:7710946-Tumor Cells, Cultured
|
|
pubmed:year |
1995
|
|
pubmed:articleTitle |
Interleukin 1 modulates growth of human renal carcinoma cells in vitro.
|
|
pubmed:affiliation |
Division of Hematology and Oncology, Technischen Universität München, Germany.
|
|
pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
|
