Linked Life Data

7708065

Source:http://linkedlifedata.com/resource/pubmed/id/7708065

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
12
pubmed:dateCreated
1995-5-9
pubmed:abstractText
The development of endocrine cell types within the pancreas is thought to involve the progressive restriction of pluripotential stem cells, which gives rise to the four major cell types: insulin-, glucagon-, somatostatin-, and pancreatic polypeptide-expressing cells. The mechanism by which these peptide hormone genes are induced and then either maintained or repressed during development is unknown, but their coexpression in early precursor cells suggests the involvement of common regulatory factors. Here we show that the somatostatin transcription factor STF-1 is also a principal regulator of insulin expression in beta-cells of the pancreas. STF-1 stimulates the insulin gene by recognizing two well defined islet-specifying elements on the insulin promoter and by subsequently synergizing in trans with the juxtaposed helix-loop-helix protein E47. Within the STF-1 protein, an N-terminal trans-activation domain functions cooperatively with E47 to stimulate insulin transcription. As truncated STF-1 polypeptides lacking the N-terminal activation domain strongly inhibit insulin promoter activity in beta-islet cells, our results suggest that the specification of islet cell types during development may be in part determined by the expression of STF-1 relative to other islet cell factors.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0888-8809
pubmed:author
pubmed:issnType
Print
pubmed:volume
8
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1798-806
pubmed:dateRevised
2011-11-17
pubmed:meshHeading
pubmed-meshheading:7708065-Animals, pubmed-meshheading:7708065-Blotting, Western, pubmed-meshheading:7708065-Cell Nucleus, pubmed-meshheading:7708065-DNA, pubmed-meshheading:7708065-DNA-Binding Proteins, pubmed-meshheading:7708065-Drug Synergism, pubmed-meshheading:7708065-Gene Expression, pubmed-meshheading:7708065-Helix-Loop-Helix Motifs, pubmed-meshheading:7708065-Homeodomain Proteins, pubmed-meshheading:7708065-Immunohistochemistry, pubmed-meshheading:7708065-Insulin, pubmed-meshheading:7708065-Islets of Langerhans, pubmed-meshheading:7708065-Mice, pubmed-meshheading:7708065-Promoter Regions, Genetic, pubmed-meshheading:7708065-TCF Transcription Factors, pubmed-meshheading:7708065-Trans-Activators, pubmed-meshheading:7708065-Transcription Factor 7-Like 1 Protein, pubmed-meshheading:7708065-Transcription Factors, pubmed-meshheading:7708065-Transcriptional Activation
pubmed:year
1994
pubmed:articleTitle
Insulin expression in pancreatic islet cells relies on cooperative interactions between the helix loop helix factor E47 and the homeobox factor STF-1.
pubmed:affiliation
Clayton Foundation Laboratories for Peptide Biology, Salk Institute, La Jolla, California 92037.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't