Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
|
| pubmed:dateCreated |
1995-5-9
|
| pubmed:abstractText |
There is accumulating evidence that cellular rather than antibody responses are more effective for tumour rejection. It is therefore important to screen anti-idiotypic (anti-id) antibodies for their ability to stimulate anti-tumour T-cell responses. The human anti-id monoclonal antibody (MAb) 105AD7 stimulated both delayed-type hypersensitivity (DTH) responses in animals and antigen-specific blastogenesis and IL-2 induction in advanced cancer patients. It may not be necessary to use human anti-id antibodies as murine anti-id antibodies, which elicit DTH responses against immunodominant human T-cell epitopes and may be just as useful in the clinic. We have therefore produced a murine anti-id antibody to the same MAb as was used to generate the human anti-id antibody and screened it for its ability to generate cellular anti-tumour immune responses. Low-dose immunization with the murine anti-id MAb NCRC60, which recognises the paratope of the anti-791Tgp72 MAb 791T/36, induced DTH responses to 791Tgp72-expressing tumour cells but not to antigen-negative cells. DTH responses with no detectable antibody responses were induced with 5 micrograms of anti-id NCRC60 without adjuvant. Addition of either complete Freund's adjuvant or Quil A did not enhance DTH responses. However, when the anti-id NCRC60 was linked to KLH and injected in the presence of Freund's adjuvant anti-anti-id antibodies and anti-791Tgp72 antibodies were induced. NCRC60 anti-id was also capable in vitro of priming human T cells from cancer patients to proliferate in response to secondary stimulation with 791Tgp72-expressing tumour cells, suggesting that it may have therapeutic potential in cancer patients.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Anti-Idiotypic,
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Monoclonal,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Neoplasm,
http://linkedlifedata.com/resource/pubmed/chemical/Glycoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/tumor-associated antigen 72
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Mar
|
| pubmed:issn |
0020-7136
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
29
|
| pubmed:volume |
61
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
62-6
|
| pubmed:dateRevised |
2007-7-24
|
| pubmed:meshHeading |
pubmed-meshheading:7705934-Animals,
pubmed-meshheading:7705934-Antibodies, Anti-Idiotypic,
pubmed-meshheading:7705934-Antibodies, Monoclonal,
pubmed-meshheading:7705934-Antigens, Neoplasm,
pubmed-meshheading:7705934-Colorectal Neoplasms,
pubmed-meshheading:7705934-Dose-Response Relationship, Drug,
pubmed-meshheading:7705934-Drug Hypersensitivity,
pubmed-meshheading:7705934-Female,
pubmed-meshheading:7705934-Glycoproteins,
pubmed-meshheading:7705934-Humans,
pubmed-meshheading:7705934-Hypersensitivity, Delayed,
pubmed-meshheading:7705934-Immunity, Cellular,
pubmed-meshheading:7705934-Immunization,
pubmed-meshheading:7705934-Lymphocyte Activation,
pubmed-meshheading:7705934-Mice,
pubmed-meshheading:7705934-Mice, Inbred BALB C,
pubmed-meshheading:7705934-Ovarian Neoplasms,
pubmed-meshheading:7705934-Stomach Neoplasms,
pubmed-meshheading:7705934-T-Lymphocytes
|
| pubmed:year |
1995
|
| pubmed:articleTitle |
Induction of cellular immune responses by a murine monoclonal anti-idiotypic antibody recognizing the 791Tgp72 antigen expressed on colorectal, gastric and ovarian human tumours.
|
| pubmed:affiliation |
Department of Surgery, University of Nottingham, UK.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|