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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
3
|
| pubmed:dateCreated |
1995-5-9
|
| pubmed:abstractText |
The rate of germ-line RNA transcription correlates with the rate of immunoglobulin heavy chain isotype switching. A promoter element for the transcription of RNA from the germ-line mouse immunoglobulin epsilon heavy chain constant region gene is induced by interleukin(IL)-4 and lipopolysaccharide, and is bound at its transcription initiation sites by an IL-4-inducible nuclear protein, NF-BRE. To examine the function of the binding site for this IL-4-inducible complex, substitution mutations were introduced in the promoter. These binding site mutations increased promoter activity and decreased binding of NF-BRE. To investigate the paradox of an IL-4-inducible protein binding to a repressor site in an IL-4-inducible promoter, we determined that the non-histone chromosomal protein HMG-I(Y) binds at the transcription initiation sites of the germ-line epsilon promoter. Assays with antisera against HMG-I(Y) revealed monomeric HMG-I(Y) in nuclear extracts. Cotransfection of an expression construct directing the synthesis of anti-sense HMG-I(Y) RNA also increased promoter activity, consistent with a repressor function of HMG-I(Y). Thus, the data are most consistent with a model in which HMG-I(Y) participates in repression of promoter activity. The effects of IL-4 may include derepression at this site.
|
| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/HMGA1a Protein,
http://linkedlifedata.com/resource/pubmed/chemical/High Mobility Group Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Immunoglobulin E,
http://linkedlifedata.com/resource/pubmed/chemical/Immunoglobulin Heavy Chains,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Proteins
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Mar
|
| pubmed:issn |
0014-2980
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
25
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
798-808
|
| pubmed:dateRevised |
2008-11-21
|
| pubmed:meshHeading |
pubmed-meshheading:7705411-Animals,
pubmed-meshheading:7705411-Base Sequence,
pubmed-meshheading:7705411-Cell Line,
pubmed-meshheading:7705411-DNA-Binding Proteins,
pubmed-meshheading:7705411-Down-Regulation,
pubmed-meshheading:7705411-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:7705411-HMGA1a Protein,
pubmed-meshheading:7705411-High Mobility Group Proteins,
pubmed-meshheading:7705411-Immunoglobulin E,
pubmed-meshheading:7705411-Immunoglobulin Heavy Chains,
pubmed-meshheading:7705411-Mice,
pubmed-meshheading:7705411-Mice, Inbred BALB C,
pubmed-meshheading:7705411-Mice, Inbred C57BL,
pubmed-meshheading:7705411-Molecular Sequence Data,
pubmed-meshheading:7705411-Mutation,
pubmed-meshheading:7705411-Promoter Regions, Genetic,
pubmed-meshheading:7705411-RNA, Messenger,
pubmed-meshheading:7705411-Recombinant Proteins,
pubmed-meshheading:7705411-Transfection
|
| pubmed:year |
1995
|
| pubmed:articleTitle |
The non-histone chromosomal protein HMG-I(Y) contributes to repression of the immunoglobulin heavy chain germ-line epsilon RNA promoter.
|
| pubmed:affiliation |
Department of Cancer Biology, Harvard School of Public Health, Boston.
|
| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
|