| pubmed-article:7704445 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:7704445 | lifeskim:mentions | umls-concept:C0007634 | lld:lifeskim |
| pubmed-article:7704445 | lifeskim:mentions | umls-concept:C0035820 | lld:lifeskim |
| pubmed-article:7704445 | lifeskim:mentions | umls-concept:C0032000 | lld:lifeskim |
| pubmed-article:7704445 | lifeskim:mentions | umls-concept:C0023610 | lld:lifeskim |
| pubmed-article:7704445 | lifeskim:mentions | umls-concept:C0033634 | lld:lifeskim |
| pubmed-article:7704445 | lifeskim:mentions | umls-concept:C0596235 | lld:lifeskim |
| pubmed-article:7704445 | lifeskim:mentions | umls-concept:C0205263 | lld:lifeskim |
| pubmed-article:7704445 | lifeskim:mentions | umls-concept:C0439799 | lld:lifeskim |
| pubmed-article:7704445 | pubmed:issue | 6 | lld:pubmed |
| pubmed-article:7704445 | pubmed:dateCreated | 1995-5-9 | lld:pubmed |
| pubmed-article:7704445 | pubmed:abstractText | The action mechanism of gonadotropin-releasing hormone (GnRH) on the cytosolic free calcium concentration ([Ca2+]i) and high-threshold voltage-dependent Ca2+ channel activity was studied in human nonsecreting (NS) pituitary adenoma cells. [Ca2+]i was monitored in individual cells by dual emission microspectrofluorimetry using indo 1 as intracellular fluorescent Ca2+ probe. The whole-cell recording patch-clamp technique was used to study Ca2+ channels. A short application of GnRH (1 to 100 nM) induced an increase in [Ca2+]i due to Ca2+ entry through plasma membrane voltage-sensitive L-type Ca2+ channels. Protein kinase C (PKC) depletion induced by a pretreatment with 1 microM PMA for 24 h abolished spontaneous Ca2+ transients and the action of GnRH on [Ca2+]i and Ca2+ channels. Phloretin (250 microM) and staurosporine (20 nM), two protein kinase C inhibitors, inhibited Ca2+ channel activity, thereby suppressing the effect of GnRH. On the other hand, activation of PKC by a short application of phorbol myristate acetate (10 nM) stimulated Ca2+ influx through Ca2+ channels. These findings demonstrate that, in human NS adenoma cells, GnRH (1 to 100 nM) induces an increase in [Ca2+]i, principally due to Ca2+ entry through L-type voltage-activated Ca2+ channels. PKC regulates this mechanism as well as basal ion channel activity, thus exerting both positive and negative control of [Ca2+]i in stimulated and unstimulated NS adenoma cells. | lld:pubmed |
| pubmed-article:7704445 | pubmed:language | eng | lld:pubmed |
| pubmed-article:7704445 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7704445 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:7704445 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7704445 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7704445 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7704445 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7704445 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:7704445 | pubmed:month | Dec | lld:pubmed |
| pubmed-article:7704445 | pubmed:issn | 1044-7431 | lld:pubmed |
| pubmed-article:7704445 | pubmed:author | pubmed-author:GuerinJJ | lld:pubmed |
| pubmed-article:7704445 | pubmed:author | pubmed-author:Dufy-BarbeLL | lld:pubmed |
| pubmed-article:7704445 | pubmed:author | pubmed-author:RivelJJ | lld:pubmed |
| pubmed-article:7704445 | pubmed:author | pubmed-author:VacherPP | lld:pubmed |
| pubmed-article:7704445 | pubmed:author | pubmed-author:OdessaM FMF | lld:pubmed |
| pubmed-article:7704445 | pubmed:author | pubmed-author:San GalliFF | lld:pubmed |
| pubmed-article:7704445 | pubmed:author | pubmed-author:SkrymaRR | lld:pubmed |
| pubmed-article:7704445 | pubmed:author | pubmed-author:PrevarskayaNN | lld:pubmed |
| pubmed-article:7704445 | pubmed:author | pubmed-author:Bresson-Bepol... | lld:pubmed |
| pubmed-article:7704445 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:7704445 | pubmed:volume | 5 | lld:pubmed |
| pubmed-article:7704445 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:7704445 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:7704445 | pubmed:pagination | 699-708 | lld:pubmed |
| pubmed-article:7704445 | pubmed:dateRevised | 2007-11-15 | lld:pubmed |
| pubmed-article:7704445 | pubmed:meshHeading | pubmed-meshheading:7704445-... | lld:pubmed |
| pubmed-article:7704445 | pubmed:meshHeading | pubmed-meshheading:7704445-... | lld:pubmed |
| pubmed-article:7704445 | pubmed:meshHeading | pubmed-meshheading:7704445-... | lld:pubmed |
| pubmed-article:7704445 | pubmed:meshHeading | pubmed-meshheading:7704445-... | lld:pubmed |
| pubmed-article:7704445 | pubmed:meshHeading | pubmed-meshheading:7704445-... | lld:pubmed |
| pubmed-article:7704445 | pubmed:meshHeading | pubmed-meshheading:7704445-... | lld:pubmed |
| pubmed-article:7704445 | pubmed:meshHeading | pubmed-meshheading:7704445-... | lld:pubmed |
| pubmed-article:7704445 | pubmed:meshHeading | pubmed-meshheading:7704445-... | lld:pubmed |
| pubmed-article:7704445 | pubmed:meshHeading | pubmed-meshheading:7704445-... | lld:pubmed |
| pubmed-article:7704445 | pubmed:meshHeading | pubmed-meshheading:7704445-... | lld:pubmed |
| pubmed-article:7704445 | pubmed:meshHeading | pubmed-meshheading:7704445-... | lld:pubmed |
| pubmed-article:7704445 | pubmed:year | 1994 | lld:pubmed |
| pubmed-article:7704445 | pubmed:articleTitle | Gonadotropin-releasing hormone induced Ca2+ influx in nonsecreting pituitary adenoma cells: role of voltage-dependent Ca2+ channels and protein kinase C. | lld:pubmed |
| pubmed-article:7704445 | pubmed:affiliation | Laboratory of Neurophysiology, University of Bordeaux II, CNRS URA 1200, France. | lld:pubmed |
| pubmed-article:7704445 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:7704445 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:7704445 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:7704445 | lld:pubmed |
