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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
1
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pubmed:dateCreated |
1995-5-9
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pubmed:abstractText |
A high-performance liquid chromatographic method with direct injection has been developed for the simultaneous determination of sulphamonomethoxine and its N4-acetyl metabolite in serum of animals and fish. A HISEP shielded hydrophobic-phase column (15 cm x 4.6 mm I.D.), a mobile phase of 0.05 M citric acid-0.2 M disodium hydrogenphosphate-acetonitrile (70:15:15, v/v), and ultraviolet detection at 265 nm were used. The standard calibration curves in serum of chicken, pig, cattle, rainbow trout and yellowtail were linear over the range 0.5-20 micrograms/ml. The recoveries of sulphamonomethoxine and its N4-acetyl metabolite from all serum samples determined at different concentrations (0.5, 2.0 and 10.0 micrograms/ml) were 93-103% and 90-103%, respectively. The lowest measurable sulphamonomethoxine and N4-acetyl metabolite concentrations were 0.04 and 0.1 microgram/ml, respectively, for all serum samples.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
1572-6495
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:day |
6
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pubmed:volume |
663
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
177-80
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pubmed:dateRevised |
2007-10-16
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pubmed:meshHeading |
pubmed-meshheading:7704208-Acetylation,
pubmed-meshheading:7704208-Animals,
pubmed-meshheading:7704208-Cattle,
pubmed-meshheading:7704208-Chickens,
pubmed-meshheading:7704208-Chromatography, High Pressure Liquid,
pubmed-meshheading:7704208-Fishes,
pubmed-meshheading:7704208-Oncorhynchus mykiss,
pubmed-meshheading:7704208-Sulfamonomethoxine,
pubmed-meshheading:7704208-Swine
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pubmed:year |
1995
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pubmed:articleTitle |
Simultaneous determination of sulphamonomethoxine and its N4-acetyl metabolite in blood serum by high-performance liquid chromatography with direct injection.
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pubmed:affiliation |
Department of Food Science, Konan Women's College, Aichi, Japan.
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pubmed:publicationType |
Journal Article
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