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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
5
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pubmed:dateCreated |
1995-5-9
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pubmed:abstractText |
Using sequential HPLC and capillary electrophoresis (CE), testibumin (CMB-1) has been purified to apparent homogeneity from Sertoli cell-enriched culture medium prepared from 20-day-old rat testes. N-Terminal amino acid sequence analysis of the purified testibumin revealed a partial sequence of NH2-XPVQDPKI. When this partial sequence was compared to existing protein database, it was shown that it is identical to a previously isolated Sertoli cell secretory protein, sulfated glycoprotein I (SGP-1). The fact that testibumin is equivalent to SGP-1 was further confirmed when its full-length cDNA was isolated and sequenced. Studies using quantitative PCR to examine the changes of steady-state mRNA level of testibumin (SGP-1) in the rat testes between 3 and 60 days of age indicated that its mRNA increased rapidly after birth, peaked at 10-20 days, and declined rapidly where the adult testibumin mRNA level was similar to the neonatal rat at 3 days of age. Depletion of germ cells by a single dose of lonidamine, an antispermatogenic drug, did not induce an increase in testibumin (SGP-1) mRNA level indicating its mRNA expression is not dependent on germ cells.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antispermatogenic Agents,
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Complementary,
http://linkedlifedata.com/resource/pubmed/chemical/Glycoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/Indazoles,
http://linkedlifedata.com/resource/pubmed/chemical/Psap protein, rat,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Saposins,
http://linkedlifedata.com/resource/pubmed/chemical/lonidamine
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
1039-9712
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
34
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1063-71
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:7703902-Aging,
pubmed-meshheading:7703902-Amino Acid Sequence,
pubmed-meshheading:7703902-Animals,
pubmed-meshheading:7703902-Antispermatogenic Agents,
pubmed-meshheading:7703902-Base Sequence,
pubmed-meshheading:7703902-Chromatography, High Pressure Liquid,
pubmed-meshheading:7703902-Cloning, Molecular,
pubmed-meshheading:7703902-DNA, Complementary,
pubmed-meshheading:7703902-Electrophoresis,
pubmed-meshheading:7703902-Gene Expression Regulation,
pubmed-meshheading:7703902-Glycoproteins,
pubmed-meshheading:7703902-Indazoles,
pubmed-meshheading:7703902-Male,
pubmed-meshheading:7703902-Molecular Sequence Data,
pubmed-meshheading:7703902-RNA, Messenger,
pubmed-meshheading:7703902-Rats,
pubmed-meshheading:7703902-Rats, Sprague-Dawley,
pubmed-meshheading:7703902-Saposins,
pubmed-meshheading:7703902-Sertoli Cells,
pubmed-meshheading:7703902-Spermatozoa,
pubmed-meshheading:7703902-Testis
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pubmed:year |
1994
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pubmed:articleTitle |
Rat testicular testibumin is identical to sulfated glycoprotein-1 (SGP-1) whose mRNA expression in the testis is age- but not germ cell-dependent.
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pubmed:affiliation |
Population Council, Center for Biomedical Research, New York, New York 10021.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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