Linked Life Data

7701867

Source:http://linkedlifedata.com/resource/pubmed/id/7701867

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
7-8
pubmed:dateCreated
1995-5-4
pubmed:abstractText
Conditions were established to optimize the lymphocyte proliferation test in sheep, a species where proliferation under standard conditions is lower than for other species. Optimum proliferation of phytohemagglutinin-stimulated lymphocytes was achieved in TCM-199 supplemented with 5% (v/v) horse serum, 0.2 mM sodium pyruvate and 5 x 10(-5) M beta-mercaptoethanol. Proliferative responses in this medium were 2.5-fold higher or more than when using a standard medium (RPMI-1640 + 10% heat-inactivated fetal bovine serum). Lymphocytes cultured in medium containing horse serum also responded to concanavalin A as well as or better than lymphocytes cultured in medium with bovine serum. For both PHA and Con A, optimal incorporation of radioactivity was achieved when cells were pulsed with [3H-methyl]-thymidine 72 h after addition of mitogen. The medium based on TCM-199 + horse serum could support interleukin-2-induced proliferation. Furthermore, known inhibitors of lymphocyte proliferation in sheep were effective in inhibiting lymphocytes cultured in this medium.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0514-7166
pubmed:author
pubmed:issnType
Print
pubmed:volume
41
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
541-7
pubmed:dateRevised
2008-2-13
pubmed:meshHeading
pubmed:year
1994
pubmed:articleTitle
Enhancement of mitogen-induced lymphocyte proliferation in sheep.
pubmed:affiliation
Department of Dairy and Poultry Sciences, University of Florida, Gainesville 32611-0920.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.