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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
1995-4-28
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pubmed:abstractText |
Xenopus oocytes expressed kappa-opioid specific binding sites after injection of cRNA prepared from a clone of the rat kappa-opioid receptor. Coinjection of kappa receptor cRNA with cRNA coding for a G protein-linked, inwardly rectifying, K+ channel (GIRK1, or KGA) resulted in oocytes that responded to the kappa agonist U-69593 by activating a large (1.0-1.5-microA) K+ current. U-69593 exhibited an EC50 of 260 +/- 50 nM and was blocked by the opioid antagonists norbinaltorphimine and naloxone. The kappa agonist bremazocine was 200-fold more potent than U-69593 in eliciting K+ current but exhibited a partial agonist profile in this expression system. The present results indicate that stimulation of inwardly rectifying K+ channels may be a potential effector mechanism for kappa-opioid receptors.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Benzeneacetamides,
http://linkedlifedata.com/resource/pubmed/chemical/GTP-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Naltrexone,
http://linkedlifedata.com/resource/pubmed/chemical/Potassium Channels,
http://linkedlifedata.com/resource/pubmed/chemical/Pyrrolidines,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Complementary,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Opioid, kappa,
http://linkedlifedata.com/resource/pubmed/chemical/U 69593,
http://linkedlifedata.com/resource/pubmed/chemical/norbinaltorphimine
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0026-895X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
47
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
551-7
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:7700253-Amino Acid Sequence,
pubmed-meshheading:7700253-Animals,
pubmed-meshheading:7700253-Base Sequence,
pubmed-meshheading:7700253-Benzeneacetamides,
pubmed-meshheading:7700253-Binding Sites,
pubmed-meshheading:7700253-Cloning, Molecular,
pubmed-meshheading:7700253-Electrophysiology,
pubmed-meshheading:7700253-Female,
pubmed-meshheading:7700253-GTP-Binding Proteins,
pubmed-meshheading:7700253-Ion Channel Gating,
pubmed-meshheading:7700253-Molecular Sequence Data,
pubmed-meshheading:7700253-Naltrexone,
pubmed-meshheading:7700253-Oocytes,
pubmed-meshheading:7700253-Potassium Channels,
pubmed-meshheading:7700253-Pyrrolidines,
pubmed-meshheading:7700253-RNA, Complementary,
pubmed-meshheading:7700253-Rats,
pubmed-meshheading:7700253-Receptors, Opioid, kappa,
pubmed-meshheading:7700253-Xenopus
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pubmed:year |
1995
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pubmed:articleTitle |
Kappa-opioid receptors couple to inwardly rectifying potassium channels when coexpressed by Xenopus oocytes.
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pubmed:affiliation |
University of Washington, Department of Pharmacology, Seattle 98195.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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