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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
12
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pubmed:dateCreated |
1995-5-3
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pubmed:abstractText |
We have established three lymphoma cell lines, HF-1 from one follicular lymphoma (FL) patient, and HF-4 and HF-9 from another. All cell lines carry the characteristic t(14;18) chromosomal translocation and express constitutively the bcl-2 gene product (Bcl-2 protein). Cross-linking of their surface membrane Igs (sIgs) with relevant antibodies triggers a vigorous calcium signal in all three lines but only HF-1 is induced to apoptosis. Treatment with anti-Ig arrests the proliferation of HF-1 within 6-12 h, nucleosomal DNA fragmentation is evident in 18 h and a morphologically complete apoptosis is seen in 24-48 h. While bcl-2 was expressed at equal levels in all lines, the apoptosis-sensitive HF-1 line displayed a much lower expression of c-myc than seen in the apoptosis-resistant line. This finding challenges the concept that expression of bcl-2 per se renders resistance to apoptosis but that the balance between the expression of bcl-2 and c-myc may dictate the outcome of sIg cross-linking. HF-1 is a unique, phenotypically mature human B cell line expressing surface IgG. This cell line offers a new tool for investigations on apoptosis and induction of tolerance in mature B lymphocytes. Our results suggest that some FLs may be amenable to anti-cancer treatment based on anti-sIg antibody induced apoptosis.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Calcium,
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Neoplasm,
http://linkedlifedata.com/resource/pubmed/chemical/Immunoglobulin G,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins c-bcl-2
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0953-8178
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
6
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pubmed:geneSymbol |
bcl-2,
c-myc
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1817-27
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:7696202-Apoptosis,
pubmed-meshheading:7696202-Calcium,
pubmed-meshheading:7696202-Cross Reactions,
pubmed-meshheading:7696202-DNA, Neoplasm,
pubmed-meshheading:7696202-Electrophoresis, Agar Gel,
pubmed-meshheading:7696202-Genes, myc,
pubmed-meshheading:7696202-Humans,
pubmed-meshheading:7696202-Immunoglobulin G,
pubmed-meshheading:7696202-Lymphocyte Activation,
pubmed-meshheading:7696202-Lymphoma, B-Cell,
pubmed-meshheading:7696202-Lymphoma, Follicular,
pubmed-meshheading:7696202-Proto-Oncogene Proteins,
pubmed-meshheading:7696202-Proto-Oncogene Proteins c-bcl-2,
pubmed-meshheading:7696202-Signal Transduction,
pubmed-meshheading:7696202-Tumor Cells, Cultured
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pubmed:year |
1994
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pubmed:articleTitle |
Cross-linking of surface IgG induces apoptosis in a bcl-2 expressing human follicular lymphoma line of mature B cell phenotype.
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pubmed:affiliation |
Department of Bacteriology and Immunology, University of Helsinki, Finland.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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