Linked Life Data

7694598

Source:http://linkedlifedata.com/resource/pubmed/id/7694598

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
11
pubmed:dateCreated
1994-1-6
pubmed:abstractText
New results are presented on the equilibria and kinetics of the ion exchange onto CM-Sephadex of polygalacturonase (pectinase) produced by the fermentation of Kluyveromyces marxianus. It is found that the equilibrium behavior follows the form of the Langmuir isotherm; the equilibrium is strongly affected by pH. High partitioning onto the ion-exchange matrix, with good retention of enzyme activity, is achieved in the pH range 3.5-5.0, and this can be qualitatively explained in terms of simple models for protein adsorption by ion exchange. The kinetics of ion exchange is modeled by assuming that the transfer resistances can be lumped into a single coefficient, and the results show that this gives a reasonable description of the adsorption kinetics. Under optimum conditions protein adsorption is enhanced by electrostatic effects and is extremely fast, and it is suggested that in these circumstances external mass transfer resistance is significant. At pH values close to the isoelectric point, electrostatic interactions are weak and intraparticle diffusion is rate-limiting: pore-blocking by adsorbed proteins appears to be important under these conditions. The results also provide the basis for an efficient single-step purification scheme.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
B
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0141-0229
pubmed:author
pubmed:issnType
Print
pubmed:volume
15
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
906-15
pubmed:dateRevised
2000-12-18
pubmed:meshHeading
pubmed:year
1993
pubmed:articleTitle
Adsorption of Kluyveromyces marxianus pectinase on CM-Sephadex gels.
pubmed:affiliation
Department of Food Science and Technology, University of Reading, UK.
pubmed:publicationType
Journal Article